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首页> 美国卫生研究院文献>Journal of Biomolecular Techniques : JBT >Enabling Technologies at the Expression Analysis and DNA Technologies Core Facilities at the UC Davis Genome Center
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Enabling Technologies at the Expression Analysis and DNA Technologies Core Facilities at the UC Davis Genome Center

机译：加州大学戴维斯分校基因组中心的表达分析和DNA技术核心设施中的辅助技术

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>CF-34The UC Davis Genome Center (GC) opened several years ago in a state-of-the-art facility and combines bench science with computational approaches to investigate questions in genomics. An important part of the GC is its five technology service cores providing access to enabling technologies to researchers within and outside the UC Davis campus. The DNA Technologies and Expression Analysis Cores are linked entities providing numerous services related to genotyping, genome structure, and sequencing. We operate three Illumina Genome Analyzer high throughput sequencers and two paired end modules. These instruments have been involved in numerous projects, including ChIP-seq, mRNA-seq, de novo genome assembly, and resequencing for SNP discovery. The Bioinformatics Core, another service entity associated with the GC, has been invaluable in rapidly allowing this instrumentation to provide full research value to the UC Davis campus. The DNA Technologies Core also utilizes all the available Illumina platforms for SNP genotyping, including the Golden Gate assay on both the iScan and BeadXpress, and the Infinium assay with associated Tecan liquid handling capability. The Expression Analysis Core provides array services and analysis on a subset of the available commercial platforms, focusing our efforts on Illumina expression arrays, as well as on NimbleGen and Agilent custom arrays. Because of local scientific expertise provided in the GC there is an emphasis on services related to chromatin immunoprecipitation (ChIP) techniques, a methodology generally unavailable in most technology service cores. We offer hands-on workshops in the basic manipulations required for ChIP as well as carrying out ChIP as a service with user-provided cells and antibodies. More information is available at .
机译：> CF-34 加州大学戴维斯分校的基因组中心（GC）于几年前在最先进的设施中开业，并将基准科学与计算方法相结合，以研究基因组学中的问题。 GC的重要组成部分是其五个技术服务核心，可为UC Davis校园内外的研究人员提供使能技术的途径。 DNA技术和表达分析核心是链接的实体，可提供与基因分型，基因组结构和测序有关的众多服务。我们运营着三个Illumina Genome Analyzer高通量测序仪和两个配对的末端模块。这些仪器已参与许多项目，包括ChIP-seq，mRNA-seq，从头基因组组装和SNP发现的重测序。生物信息学核心是与GC相关的另一个服务实体，在迅速使该仪器为UC Davis校园提供全面研究价值方面具有不可估量的价值。 DNA Technologies Core还利用所有可用的Illumina平台进行SNP基因分型，包括iScan和BeadXpress上的金门测定法以及具有相关Tecan液体处理能力的Infinium测定法。 Expression Analysis Core在一部分可用的商业平台上提供阵列服务和分析，将我们的工作重点放在Illumina表达阵列以及NimbleGen和Agilent定制阵列上。由于GC中提供了本地科学专业知识，因此重点关注与染色质免疫沉淀（ChIP）技术相关的服务，而该技术通常是大多数技术服务核心所不具备的。我们提供有关ChIP所需的基本操作的动手研讨会，并通过用户提供的细胞和抗体将ChIP作为一项服务来进行。有关更多信息，请访问。

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