• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Animal Science >ASAS-SSR Triennial Reproduction Symposium: The use of natural cycle’s follicular dynamic to improve oocyte quality in dairy cows and heifers
【2h】

ASAS-SSR Triennial Reproduction Symposium: The use of natural cycle’s follicular dynamic to improve oocyte quality in dairy cows and heifers

机译：ASAS-SSR三年期繁殖研讨会：利用自然周期的卵泡动力学改善奶牛和小母牛的卵母细胞质量

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The selection of the best dairy heifers is mainly driven by the genetic value of their parents. The phenotype analysis of cows and of the daughters of bulls has been used to identify the best genetic value for decades before being replaced by genomic selection of individuals that are not yet parents. Because it is possible to predict the future value of an individual by its genetic makeup, it becomes feasible to do it as early as the blastocyst stage and to decide which should be transferred or not. Because we know the genotype of an animal at birth, or even before, it is becoming desirable to reproduce this animal as soon as possible to reduce generation interval and improve selection speed. Nature provides constraints that can be overcome: a single oocyte per cycle and age at puberty. Indeed, it is now possible to super-stimulate the ovary at any age and to start collecting oocytes at 6 mo by trans-vaginal ultrasonography. The challenge becomes the production of good eggs and embryos capable of implanting and developing into healthy calves. Our understanding of ovarian follicular physiology has been instrumental in designing stimulation protocols that may be adjusted to any physiological context including age, and even the individual animal, to obtain a good response. Therefore, the combination of procedures developed in cows to optimize oocyte quality, for example, FSH coasting, in association with in vitro fertilization and optimal culture conditions can now result in the production of several female embryos twice a month from animals 6 to12 mo of age. The transcriptomic and epigenetic analyses of embryos produced from the same females at different ages were compared and few differences were noted in particular in relation to embryo metabolism. These embryos are as good as the ones obtained from adult animals and can be produced with sexed sperm of bulls 12 mo of age. This combination of these technical optimizations with blastocyst genotyping allows the selection of a second generation within a year.
机译：最佳奶牛的选择主要取决于其父母的遗传价值。在被尚未成亲的个体的基因组选择所取代之前，对母牛和公牛的女儿的表型分析已被用来确定数十年来的最佳遗传价值。由于可以通过其遗传构成来预测一个人的未来价值，因此早在胚泡阶段就可以确定一个人的未来价值，并决定是否应该转移它。因为我们知道动物在出生时或什至之前的基因型，所以希望尽快繁殖该动物以减少世代间隔并提高选择速度。大自然提供了可以克服的限制：每个周期和青春期的单个卵母细胞。确实，现在有可能在任何年龄对卵巢进行超刺激，并通过经阴道超声检查在6 mo时开始收集卵母细胞。面临的挑战是如何生产能够植入并发育为健康小牛的优质卵和胚胎。我们对卵巢卵泡生理的理解有助于设计刺激方案，可以将其调整至包括年龄甚至任何动物在内的任何生理环境以获得良好的反应。因此，结合奶牛开发的优化卵母细胞质量的程序（例如FSH滑行），再加上体外受精和最佳培养条件，现在可以每月从6至12月龄的动物中产生两次雌性胚胎。比较了从不同年龄的同一雌性产生的胚胎的转录组和表观遗传学分析，特别是在胚胎代谢方面，几乎没有差异。这些胚胎与从成年动物获得的胚胎一样好，并且可以用12个月以上的公牛的有性精子生产。这些技术优化与胚泡基因分型的结合，使得一年内可以选择第二代。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号