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首页> 美国卫生研究院文献>Journal of Animal Science >Genetic relationships of antibody response viremia level and weight gain in pigs experimentally infected with porcine reproductive and respiratory syndrome virus
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Genetic relationships of antibody response viremia level and weight gain in pigs experimentally infected with porcine reproductive and respiratory syndrome virus

机译：实验性感染猪繁殖与呼吸综合征病毒的猪的抗体应答病毒血症水平和体重增加的遗传关系

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Genetic and antigenic variability between Porcine Reproductive and Respiratory Syndrome Virus (>PRRSV) isolates has encumbered vaccine development. Here, the genetic basis of PRRSV antibody response was assessed using data from experimental infection trials of commercial crossbred weaner pigs across with one of two distinct PRRSV isolates, NVSL-97–7895 (~750 pigs) and KS-2006–72109 (~450 pigs). Objectives were to estimate the genetic parameters of antibody response, measured as the sample to positive ratio (>S:P) of PRRSV N-protein specific IgG in serum at 42 d post infection (dpi); assess the relationship of S:P at 42 dpi with serum viremia and growth under infection; and identify genomic regions associated with S:P at 42 dpi. Estimates of heritability of S:P at 42 dpi for NVSL and KS06 were 0.31 ± 0.09 and 0.40 ± 0.10 and appeared to be under similar genetic control (genetic correlation 0.73 ± 0.39). Estimates of genetic correlations of S:P were generally weak with viral load (NVSL: −0.20 ± 0.18; KS06: −0.69 ± 0.20), measured as area under the curve of log10 serum viremia from 0 to 21 dpi, and with weight gain (>WG) from 0 to 42 dpi (NVSL: −0.38 ± 0.19; KS06: −0.08 ± 0.25). However, genetic correlations of S:P at 42 dpi with daily serum viremia and with 3-d WG revealed dynamic relationships, with S:P at 42 dpi having the strongest negative genetic correlations with daily viremia when IgG production starts (10–20 dpi), and negative genetic correlations with WG early after infection but positive later on. This suggests that animals that placed more emphasis on immune response early in infection reaped benefits of that later in infection by more effectively clearing the virus. The WUR10000125 SNP on SSC4, previously associated with response to PRRSV, did not have a significant effect on S:P at 42 dpi (P > 0.05) but genotype-specific genetic correlations of S:P with daily viremia and 3-d WG suggested that the lower WG of pigs with the unfavorable AA WUR10000125 genotype may be due to their utilization of a more energetically costly host response compared to pigs with the favorable genotype. Genome-wide association studies identified three SNPs in the Major Histocompatibility Complex associated with S:P that explained ~10 (NVSL) and 45% (KS06) of the genetic variance but were not associated with viremia or WG. In conclusion, antibody response to PRRSV infection is a possible biomarker for improved host response to PRRSV infection.
机译：猪繁殖与呼吸综合症病毒（> PRRSV ）分离株之间的遗传和抗原变异性阻碍了疫苗的开发。在这里，PRRSV抗体应答的遗传基础是根据商品杂交断奶仔猪与两种不同PRRSV分离株之一NVSL-97–7895（〜750头）和KS-2006–72109（〜450头）的实验感染试验中的数据进行评估的猪）。目的是评估抗体反应的遗传参数，以感染后42 d（dpi）血清中PRRSV N蛋白特异性IgG的样品与阳性比率（> S：P ）进行测量；评估42 dpi的S：P与血清病毒血症和感染下生长的关系；并确定与42 dpi的S：P相关的基因组区域。 NVSL和KS06在42 dpi时S：P的遗传力估计为0.31±0.09和0.40±0.10，并且似乎处于相似的遗传控制之下（遗传相关系数0.73±0.39）。 S：P的遗传相关性估计通常在病毒载量（NVSL：-0.20±0.18; KS06：-0.69±0.20），从0到21 dpi的log10血清病毒血症曲线下面积和体重增加的情况下较弱（> WG ）从0到42 dpi（NVSL：-0.38±0.19； KS06：-0.08±0.25）。但是，42 dpi的S：P与每日血清病毒血症和3-d WG的遗传相关性显示出动态关系，当IgG产生开始时，42 dpi的S：P与每日病毒血症的负遗传相关性最强（10–20 dpi ），并且感染后与WG的遗传相关性为负，但后来呈阳性。这表明在感染初期更加重视免疫反应的动物通过更有效地清除病毒而获得了后期感染的益处。 SSC4上的WUR10000125 SNP先前与PRRSV的反应有关，在42 dpi时对S：P没有显着影响（P> 0.05），但建议S：P与日常病毒血症和3-d WG的基因型特异性遗传相关AA WUR10000125基因型不利的猪的WG较低，可能是由于与具有良好基因型的猪相比，它们利用了能量消耗更大的宿主反应。全基因组关联研究在与S：P相关的主要组织相容性复合物中鉴定了三个SNP，它们解释了〜10（NVSL）和45％（KS06）的遗传变异，但与病毒血症或WG不相关。总之，对PRRSV感染的抗体反应可能是改善宿主对PRRSV感染的反应的生物标志物。

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