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Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene.

机译：肺炎链球菌异源双链DNA错配修复系统：hexA基因的克隆和表达。

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摘要

Mutations affecting heteroduplex DNA mismatch repair in Streptococcus pneumoniae were localized in two genes, hexA and hexB, by fractionation of restriction fragments carrying mutant alleles. A fragment containing the hexA4 allele was cloned in the S. pneumoniae cloning system, and the hexA+ allele was introduced into the recombinant plasmid by chromosomal facilitation of plasmid transfer. Subcloning localized the functional hexA gene to a 3.5-kilobase segment of the cloned pneumococcal DNA. The product of this gene was shown in Bacillus subtilis minicells to be a polypeptide with an Mr of 86,000. Two mutant alleles of hexA showed partial expression of the repair system when present in multicopy plasmids. A model for mismatch repair, which depends on the interaction of two protein components to recognize the mismatched base pair and excise a segment of DNA between strand breaks surrounding the mismatch, is proposed.

机译：通过分离携带突变等位基因的限制性片段，影响肺炎链球菌异源双链DNA错配修复的突变位于两个基因hexA和hexB中。将含有hexA4等位基因的片段克隆到肺炎链球菌克隆系统中，并通过染色体促进质粒转移将hexA +等位基因引入重组质粒。亚克隆将功能性hexA基因定位在克隆的肺炎球菌DNA的3.5碱基碱基的片段上。该基因的产物在枯草芽孢杆菌微细胞中显示为Mr为86,000的多肽。当存在于多拷贝质粒中时，hexA的两个突变等位基因显示出修复系统的部分表达。提出了一种错配修复模型，该模型依赖于两个蛋白质组分的相互作用来识别错配的碱基对，并切除围绕错配的链断裂之间的一段DNA。

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期刊名称 Journal of Bacteriology
作者
T S Balganesh; S A Lacks;
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作者单位

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年(卷),期 1985(162),3
年度 1985
页码 979–984
总页数 6
原文格式 PDF
正文语种
中图分类微生物学;
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专利

1. Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene. [J] . T S Balganesh, S A Lacks Journal of bacteriology . 1985,第3期

机译：肺炎链球菌异源双链DNA错配修复系统：hexA基因的克隆和表达。
2. Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene. [J] . T S Balganesh, S A Lacks Journal of bacteriology . 1985,第3期

机译：肺炎链球菌异源双链DNA错配修复系统：hexA基因的克隆和表达。
3. Nucleotide sequence of the hexA gene for DNA mismatch repair in Streptococcus pneumoniae and homology of hexA to mutS of Escherichia coli and Salmonella typhimurium. [J] . S D Priebe, S M Hadi, B Greenberg, Journal of bacteriology . 1988,第1期

机译：用于肺炎链球菌DNA错配修复的hexA基因的核苷酸序列，以及hexA与大肠杆菌和鼠伤寒沙门氏菌mutS的同源性。
4. Application of Quaternion in Improving the Quality of Global Sequence Alignment Scores for an Ambiguous Sequence Target in Streptococcus Pneumoniae DNA [C] . D. Lestari, A. Bustamam, T. Novianti, International Symposium on Current Progress in Mathematics and Sciences . 2017

机译：四元素在肺炎链球菌DNA中提高全局序列对准分数质量的应用
5. Studies of schistosome DNA: Species and strain differences from melting and drug resistance DNA analysis and cloning, sequencing and expression analysis of a putative praziquantel binding Ca(++)-ATPase gene. [D] . Tsai, Meng-Hsiun. 1998

机译：血吸虫DNA的研究：物种和菌株差异从融化和耐药性DNA分析以及推定的吡喹酮结合Ca（++）-ATPase基因的克隆，测序和表达分析。
6. Nucleotide sequence of the hexA gene for DNA mismatch repair in Streptococcus pneumoniae and homology of hexA to mutS of Escherichia coli and Salmonella typhimurium. [O] . S D Priebe, S M Hadi, B Greenberg, 1988

机译：用于肺炎链球菌DNA错配修复的hexA基因的核苷酸序列以及hexA与大肠杆菌和鼠伤寒沙门氏菌mutS的同源性。
7. Nucleotide sequence of the hexA gene for DNA mismatch repair in Streptococcus pneumoniae and homology of hexA to mutS of Escherichia coli and Salmonella typhimurium. [O] . Priebe, S D, Hadi, S M, Greenberg, B, 1988

机译：用于肺炎链球菌DNA错配修复的hexA基因的核苷酸序列，以及hexA与大肠杆菌和鼠伤寒沙门氏菌mutS的同源性。
8. Generalized DNA Mismatch Repair: Its Molecular Basis in Streptococcus Pneumoniae and Other Organisms. [R] . Lacks, S. A. 1988

机译：广义DNa错配修复：其在肺炎链球菌和其他生物体中的分子基础。

Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene.

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