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首页> 美国卫生研究院文献>Journal of Bacteriology >Concentrations of 4.5S RNA and Ffh protein in Escherichia coli: the stability of Ffh protein is dependent on the concentration of 4.5S RNA.
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Concentrations of 4.5S RNA and Ffh protein in Escherichia coli: the stability of Ffh protein is dependent on the concentration of 4.5S RNA.

机译：大肠杆菌中4.5S RNA和Ffh蛋白的浓度：Ffh蛋白的稳定性取决于4.5S RNA的浓度。

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We measured the concentrations of both 4.5S RNA and Ffh protein under a variety of growth conditions and found that there were 400 molecules of 4.5S RNA per 10,000 ribosomes in wild-type cells and that the concentration of Ffh protein was one-fourth of that. This difference in concentration is 1 order of magnitude less than that previously reported but still significant. Pulse-chase labeling experiments indicated that Ffh protein is unstable in cells carrying ffh on high-copy-number plasmids and that simultaneous overproduction of 4.5S RNA stabilizes Ffh protein. Our analyses show that free Ffh protein is degraded with a half-life of approximately 20 min. We also tested whether three previously isolated suppressors of 4.5S RNA deficiency could reduce the requirement for Ffh protein. Since the two sffE suppressors do not suppress the Ffh requirement, we suggest that 4.5S RNA either acts in a sequential reaction with Ffh or has two functions.
机译：我们在各种生长条件下测量了4.5S RNA和Ffh蛋白的浓度，发现野生型细胞中每10,000个核糖体中有400分子4.5S RNA，Ffh蛋白的浓度是该分子的四分之一。 。这种浓度差异比以前报道的小1个数量级，但仍然很明显。脉冲追踪标记实验表明，Ffh蛋白在高拷贝数质粒上带有ffh的细胞中不稳定，同时产生的4.5S RNA过量会使Ffh蛋白稳定。我们的分析表明，游离Ffh蛋白的降解半衰期约为20分钟。我们还测试了三种先前分离的4.5S RNA缺乏抑制剂是否可以减少Ffh蛋白的需求。由于这两个sffE抑制剂不能抑制Ffh的需求，因此我们建议4.5S RNA要么与Ffh发生顺序反应，要么具有两个功能。

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