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首页> 美国卫生研究院文献>Journal of Bacteriology >Regulation of VanB-type vancomycin resistance gene expression by the VanS(B)-VanR (B) two-component regulatory system in Enterococcus faecalis V583.
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Regulation of VanB-type vancomycin resistance gene expression by the VanS(B)-VanR (B) two-component regulatory system in Enterococcus faecalis V583.

机译：粪肠球菌V583中的VanS（B）-VanR（B）两组分调节系统可调节VanB型万古霉素抗性基因表达。

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Acquired VanA- and VanB-type glycopeptide resistance in enterococci is due to synthesis of modified peptidoglycan precursors terminating in D-lactate. As opposed to VanA-type strains which are resistant to both vancomycin and teicoplanin, VanB-type strains remain teicoplanin susceptible. We have determined the sequence of a 7,160-bp DNA fragment associated with VanB-type resistance in Enterococcus faecalis V583 that contains seven open reading frames. The distal part encoded the VanH (B), VanB, and VanX (B) proteins that are highly similar to the putative VanH, VanA, and VanX proteins responsible for VanA-type resistance. Upstream from the structural genes for these proteins were the vanY(B) gene encoding a D,D-carboxypeptidase and an open reading frame vanW with an unknown function. The proximal part of the gene cluster coded for the apparent VanS(B)-VanR (B) two-component regulatory system. VanR (B) was related to response regulators of the OmpR subclass, and VanS (B) was related to membrane-associated histidine protein kinases. Analysis of transcriptional fusions with a reporter gene and promoter mapping indicated that the VanR B-VanS B two-component regulatory system activates a promoter located immediately downstream from the vanS B gene. Vancomycin, but not teicoplanin, was an inducer, which explains teicoplanin susceptibility of VanB-type enterococci.
机译：肠球菌中获得的VanA型和VanB型糖肽耐药性是由于终止于D-乳酸的修饰肽聚糖前体的合成所致。与对万古霉素和替考拉宁都具有抗性的VanA型菌株相反，VanB型菌株仍对替考拉宁敏感。我们已经确定了与粪肠球菌V583中的VanB型抗性相关的7,160 bp DNA片段的序列，该序列包含七个开放阅读框。远端部分编码VanH（B），VanB和VanX（B）蛋白，与负责VanA型耐药的推定VanH，VanA和VanX蛋白高度相似。这些蛋白质的结构基因上游是编码D，D-羧肽酶的vanY（B）基因和功能未知的开放阅读框vanW。基因簇的近端部分编码为明显的VanS（B）-VanR（B）两组分调节系统。 VanR（B）与OmpR亚类的反应调节因子有关，而VanS（B）与膜相关的组氨酸蛋白激酶有关。具有报道基因的转录融合和启动子作图的分析表明，VanR B-VanS B两组分调节系统激活了位于vanS B基因下游的启动子。万古霉素而不是替考拉宁是诱导剂，这解释了替考拉宁对VanB型肠球菌的敏感性。

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