• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Bacteriology >A Molecular Switch Controlling Competence and Motility: Competence Regulatory Factors ComS MecA and ComK Control ςD-Dependent Gene Expression in Bacillus subtilis
【2h】

A Molecular Switch Controlling Competence and Motility: Competence Regulatory Factors ComS MecA and ComK Control ςD-Dependent Gene Expression in Bacillus subtilis

机译：控制能力和动力的分子开关：能力调节因子ComSMecA和ComK控制枯草芽孢杆菌中依赖D的基因表达。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacillus subtilis, like many bacteria, will choose among several response pathways when encountering a stressful environment. Among the processes activated under growth-restricting conditions are sporulation, establishment of motility, and competence development. Recent reports implicate ComK and MecA-ClpC as part of a system that regulates both motility and competence development. MecA, while negatively controlling competence by inhibiting ComK, stimulates ςD-dependent transcription of genes that function in motility and autolysin production. Both ComK-dependent and -independent pathways have been proposed for MecA’s role in the regulation of motility. Mutations in mecA reduce the transcription of hag. encoding flagellin, and are partially suppressed by comK in both medium promoting motility and medium promoting competence. Reduced ςD levels are observed in mecA mutants grown in competence medium, but no change in ςD concentration is detected in a comK mutant. The comF operon, transcription of which requires ComK, is located immediately upstream of the operon that contains the flgM gene, encoding the ςD-specific antisigma factor. An insertion mutation that disrupts the putative comF-flgM transcription unit confers a phenotype identical to that of the comK mutant with respect to hag-lacZ expression. Expression of a flgM-lacZ operon fusion is reduced in both sigD and comK mutant cells but is abolished in the sigD comK double mutant. Reverse transcription-PCR examination of the comF-flgM transcript indicates that readthrough from comF into the flgM operon is dependent on ComK. ComK negatively controls the transcription of hag by stimulating the transcription of comF-flgM, thereby increasing the production of the FlgM antisigma factor that inhibits ςD activity. There likely exists another comK-independent mechanism of hag transcription that requires mecA and possibly affects the ςD concentration in cells undergoing competence development.
机译：像许多细菌一样，枯草芽孢杆菌在遇到压力环境时会在几种反应途径中进行选择。在限制生长的条件下激活的过程包括孢子形成，能动性的建立和能力发展。最近的报道暗示ComK和MecA-ClpC是调节运动性和能力发展的系统的一部分。 MecA虽然可以通过抑制ComK来负面地控制能力，但可以刺激Î D 依赖性基因的转录，这些基因在运动性和自溶素产生中起作用。已经提出了依赖于ComK的途径和独立于非途径的途径，以证明MecA在运动调节中的作用。 mecA中的突变减少了hag的转录。编码鞭毛蛋白，并且在促进运动的培养基和促进能力的培养基中均被comK部分抑制。在感受态培养基中生长的mecA突变体中观察到ς D 水平降低，但是在comK突变体中未检测到ς D 浓度变化。转录需要ComK的comF操纵子位于操纵子的上游，该操纵子包含flgM基因，该基因编码ς D 特异性抗σ因子。在hag-lacZ表达方面，破坏假定的comF-flgM转录单位的插入突变赋予与comK突变体相同的表型。 flgM-lacZ操纵子融合体的表达在sigD和comK突变体细胞中均降低，但在sigD comK双重突变体中被废止。 comF-flgM转录本的逆转录PCR检测表明，从 comF 到 flgM 操纵子的通读依赖于ComK。 ComK通过刺激 comF-flgM 的转录来负面控制 hag 的转录，从而增加抑制ς D 的FlgM抗σ因子的产生。活动。可能存在另一种 comK 独立的 hag 转录机制，该机制需要 mecA 并可能影响cDNA中 D 的浓度细胞经历能力发展。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号