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首页> 美国卫生研究院文献>Journal of Bacteriology >Transcriptional Regulation of the Flavohemoglobin Gene for Aerobic Nitric Oxide Detoxification by the Second Nitric Oxide-Responsive Regulator of Pseudomonas aeruginosa
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Transcriptional Regulation of the Flavohemoglobin Gene for Aerobic Nitric Oxide Detoxification by the Second Nitric Oxide-Responsive Regulator of Pseudomonas aeruginosa

机译：铜绿假单胞菌的第二种一氧化氮响应调节剂对黄酮血红蛋白基因的有氧一氧化氮解毒的转录调控。

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摘要

The regulatory gene for a σ54-dependent-type transcriptional regulator, fhpR, is located upstream of the fhp gene for flavohemoglobin in Pseudomonas aeruginosa. Transcription of fhp was induced by nitrate, nitrite, nitric oxide (NO), and NO-generating reagents. Analysis of the fhp promoter activity in mutant strains deficient in the denitrification enzymes indicated that the promoter was regulated by NO or related reactive nitrogen species. The NO-responsive regulation was operative in a mutant strain deficient in DNR (dissimilatory nitrate respiration regulator), which is the NO-responsive regulator required for expression of the denitrification genes. A binding motif for σ54 was found in the promoter region of fhp, but an FNR (fumarate nitrate reductase regulator) box was not. The fhp promoter was inactive in the fhpR or rpoN mutant strain, suggesting that the NO-sensing regulation of the fhp promoter was mediated by FhpR. The DNR-dependent denitrification promoters (nirS, norC, and nosR) were active in the fhpR or rpoN mutants. These results indicated that P. aeruginosa has at least two independent NO-responsive regulatory systems. The fhp or fhpR mutant strains showed sensitivity to NO-generating reagents under aerobic conditions but not under anaerobic conditions. These mutants also showed significantly low aerobic NO consumption activity, indicating that the physiological role of flavohemoglobin in P. aeruginosa is detoxification of NO under aerobic conditions.
机译：s 54 依赖型转录调节子fhpR的调节基因位于铜绿假单胞菌黄素血红蛋白的fhp基因的上游。 fhp的转录由硝酸盐，亚硝酸盐，一氧化氮（NO）和生成NO的试剂诱导。对缺乏反硝化酶的突变菌株中fhp启动子活性的分析表明，该启动子受NO或相关活性氮物质的调节。 NO响应调节在缺乏DNR（异化硝酸盐呼吸调节剂）的突变菌株中有效，DNR是表达反硝化基因所需的NO响应调节剂。在fhp的启动子区域发现了σ 54 的结合基序，但没有FNR（富马酸酯硝酸还原酶调节剂）框。 fhp启动子在fhpR或rpoN突变株中是无活性的，这表明fhp启动子的NO感应调节是由FhpR介导的。 DNR依赖的反硝化启动子（nirS，norC和nosR）在fhpR或rpoN突变体中具有活性。这些结果表明铜绿假单胞菌具有至少两个独立的NO响应调节系统。在有氧条件下， fhp 或 fhpR 突变菌株对产生NO的试剂敏感，而在厌氧条件下则不敏感。这些突变体还显示出明显低的需氧NO消耗活性，表明黄素血红蛋白在 P中的生理作用。铜绿是有氧条件下的NO排毒。

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