Isolation identification and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers

机译：使用c-kit和PGP9.5标记物分离鉴定和培养山羊精原干细胞

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IntroductionPresently the techniques for making transgenic animals are cumbersome, required costly instruments and trained man-power. The ability of spermatogonial stem cells (SSCs) to integrate foreign genes has provided the opportunity for developing alternate methods for generation of transgenic animals. One of the big challenges in this field is development of the methods to identify and purify donor SSCs by antibody mediated cell sorting.

机译：引言目前，制造转基因动物的技术繁琐，需要昂贵的仪器和训练有素的人力。精原干细胞（SSCs）整合外源基因的能力为开发替代方法产生转基因动物提供了机会。该领域的最大挑战之一是开发通过抗体介导的细胞分选来鉴定和纯化供体SSC的方法。

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期刊名称 Journal of Assisted Reproduction and Genetics
作者
Banafsheh Heidari; Maryam Rahmati-Ahmadabadi; Mohammad Mehdi Akhondi; Amir Hassan Zarnani; Mahmood Jeddi-Tehrani; Abolfazl Shirazi; Mohammad Mehdi Naderi; Bahareh Behzadi;
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作者单位

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年(卷),期 2012(29),10
年度 2012
页码 1029–1038
总页数 10
原文格式 PDF
正文语种
中图分类遗传学;妇产科学;
关键词
PGP9.5 c-kit Goat Spermatogonia Growth factors;

机译：PGP9.5;c-kit;山羊;精原细胞;生长因子;

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专利

1. Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers [J] . HeidariB., Rahmati-AhmadabadiM., AkhondiM.M., Journal of assisted reproduction and genetics . 2012,第10期

机译：使用c-kit和PGP9.5标记物分离，鉴定和培养山羊精原干细胞
2. Isolation, identification, and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers [J] . Banafsheh Heidari, Maryam Rahmati-Ahmadabadi, Mohammad Mehdi Akhondi, Journal of Assisted Reproduction and Genetics . 2012,第10期

机译：使用c-kit和PGP9.5标记物分离，鉴定和培养山羊精原干细胞
3. Developments in techniques for the isolation, enrichment, main culture conditions and identification of spermatogonial stem cells [J] . Yanan He, Xiaoli Chen, Huabin Zhu, Cytotechnology . 2015,第6期

机译：精原干细胞的分离，富集，主要培养条件和鉴定技术的发展
4. Dynamic Compression Alters Extracellular Matrix Biosynthesis of the Goat Temporomandibular Joint Disc Cells Cultured in Agarose Gel [C] . Bao Guang-jie, Kang Hong, Ke Yi-fan 5th International Conference on Bioinformatics and Biomedical Engineering . 2011

机译：动态压缩改变了琼脂糖凝胶中培养的山羊颞下颌关节盘细胞的细胞外基质生物合成。
5. Development of culture methods from spermatogonial stem cells and ectopic testicular xenografting in the bull. [D] . Oatley, Jon Michael. 2004

机译：精原干细胞和异位睾丸异种移植技术的发展。
6. Developments in techniques for the isolation enrichment main culture conditions and identification of spermatogonial stem cells [O] . Yanan He, Xiaoli Chen, Huabin Zhu, 2015

机译：精原干细胞的分离富集主要培养条件和鉴定技术的发展
7. Spermatogonial Stem Cells in Fish: Characterization, Isolation, Enrichment, and Recent Advances of In Vitro Culture Systems [O] . Xuan Xie, Rafael Nóbrega, Martin Pšenička 2020

机译：鱼种干细胞在鱼类中：表征，分离，富集以及体外培养系统的最新进展

Isolation identification and culture of goat spermatogonial stem cells using c-kit and PGP9.5 markers

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