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首页> 美国卫生研究院文献>3 Biotech >Marker-assisted breeding for introgression of opaque-2 allele into elite maize inbred line BML-7
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Marker-assisted breeding for introgression of opaque-2 allele into elite maize inbred line BML-7

机译：标记辅助育种将不透明2等位基因渗入玉米优良自交系BML-7

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摘要

Improvement of quality protein maize (QPM) along with high content of lysine and tryptophan had foremost importance in maize breeding program. The efficient and easiest way of developing QPM hybrids was by backcross breeding in marker aided selection. Hence, the present investigation aimed at conversion of elite maize inbred line BML-7 into QPM line. CML-186 was identified to be a donor variety as it revealed high-quality polymorphism with BML-7 for opaque-2 gene specific marker umc1066. Non-QPM inbred line BML-7 was crossed with QPM donor CML-186 and produced F1 followed by the development of BC1F1 and BC2F1 population. Foreground selection was carried out with umc1066 in F1, and selected plants were used for BC1F1 and BC2F1 populations. Two hundred plants were screened in both BC1F1 and BC2F1 population with umc1066 for foreground selection amino acid modifiers. Foreground selected plants for both opaque-2 and amino acid modifiers were screened for background selection for BML-7 genome. Recurrent parent genome (RPG) was calculated for BC2F1 population plants. Two plants have shown with RPG 90–93% in two generation with back cross population. Two BC2F2 populations resulted from marker recognized BC2F1 individuals subjected toward foreground selection followed by tryptophan estimation. The tryptophan and lysine concentration was improved in all the plants. BC2F2 lines developed from hard endosperm kernels were selfed for BC2F2 lines and finest line was selected to illustrate the QPM version of BML-7, with 0.97% of tryptophan and 4.04% of lysine concentration in protein. Therefore, the QPM version of BML-7 line can be used for the development of single cross hybrid QPM maize version.
机译：在玉米育种计划中，最重要的是提高优质蛋白质玉米（QPM）的含量以及赖氨酸和色氨酸的含量。开发QPM杂种的最有效，最简单的方法是在标记辅助选择中进行回交育种。因此，本研究旨在将优良玉米自交系BML-7转化为QPM系。 CML-186被鉴定为供体品种，因为它对不透明2基因特异性标记umc1066具有BML-7的高质量多态性。非QPM自交系BML-7与QPM供体CML-186杂交并产生F1，随后发育BC1F1和BC2F1种群。在F1中使用umc1066进行前景选择，并将选定的植物用于BC1F1和BC2F1种群。使用umc1066在BC1F1和BC2F1群体中筛选了200株植物作为前景选择氨基酸修饰剂。筛选了不透明2和氨基酸修饰剂的前景选择植物，以进行BML-7基因组的背景选择。计算了BC2F1种群植物的复发亲本基因组（RPG）。在两代回交种群中，两株植物的RPG 90-93％。两个BC 2 F 2 种群是由标记识别的BC 2 F 1 个体进行前景选择，随后是色氨酸而产生的估计。所有植物的色氨酸和赖氨酸浓度均得到改善。从硬胚乳核发育的BC 2 F 2 系自交BC 2 F 2 系，最细系为被选择来说明BML-7的QPM版本，其中蛋白质的含量为0.97％的色氨酸和4.04％的赖氨酸浓度。因此，BML-7系列的QPM版本可用于开发单交杂种QPM玉米版本。

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