首页> 美国卫生研究院文献>Italian Journal of Food Safety >Inactivation of Heat Adapted and Chlorine Adapted Listeria Monocytogenes ATCC 7644 on Tomatoes Using Sodium Dodecyl Sulphate Levulinic Acid and Sodium Hypochlorite Solution
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Inactivation of Heat Adapted and Chlorine Adapted Listeria Monocytogenes ATCC 7644 on Tomatoes Using Sodium Dodecyl Sulphate Levulinic Acid and Sodium Hypochlorite Solution

机译:十二烷基硫酸钠乙酰丙酸和次氯酸钠溶液灭活番茄上的热适应性和氯适应性单核细胞增生李斯特菌ATCC 7644

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摘要

The effectiveness of sodium dodecyl sulphate (SDS), sodium hypochlorite solution and levulinic acid in reducing the survival of heat adapted and chlorine adapted Listeria monocytogenes ATCC 7644 was evaluated. The results against heat adapted L. monocytognes revealed that sodium hypochlorite solution was the least effective, achieving log reduction of 2.75, 2.94 and 3.97 log colony forming unit (CFU)/mL for 1, 3 and 5 minutes, respectively. SDS was able to achieve 8 log reduction for both heat adapted and chlorine adapted bacteria. When used against chlorine adapted L. monocytogenes sodium hypochlorite solution achieved log reduction of 2.76, 2.93 and 3.65 log CFU/mL for 1, 3 and 5 minutes, respectively. Using levulinic acid on heat adapted bacteria achieved log reduction of 3.07, 2.78 and 4.97 log CFU/mL for 1, 3, 5 minutes, respectively. On chlorine adapted bacteria levulinic acid achieved log reduction of 2.77, 3.07 and 5.21 log CFU/mL for 1, 3 and 5 minutes, respectively. Using a mixture of 0.05% SDS and 0.5% levulinic acid on heat adapted bacteria achieved log reduction of 3.13, 3.32 and 4.79 log CFU/mL for 1, 3 and 5 minutes while on chlorine adapted bacteria it achieved 3.20, 3.33 and 5.66 log CFU/mL, respectively. Increasing contact time also increased log reduction for both test pathogens. A storage period of up to 72 hours resulted in progressive log reduction for both test pathogens. Results also revealed that there was a significant difference (P≤0.05) among contact times, storage times and sanitizers. Findings from this study can be used to select suitable sanitizers and contact times for heat and chlorine adapted L. monocytogenes in the fresh produce industry.
机译:评估了十二烷基硫酸钠(SDS),次氯酸钠溶液和乙酰丙酸在降低热适应性和氯适应性单核细胞增生李斯特菌ATCC 7644的存活率方面的有效性。针对热适应性单核细胞增生李斯特菌的结果表明,次氯酸钠溶液效果最差,分别在1、3和5分钟内分别达到2.75、2.94和3.97对数菌落形成单位(CFU)/ mL的对数降低。对于热适应性细菌和氯适应性细菌,SDS均能够减少8 log。当针对适应氯气的单核细胞增生李斯特菌次氯酸钠溶液分别在1、3和5分钟内分别实现对数降低2.76、2.93和3.65 log CFU / mL。在热适应性细菌上使用乙酰丙酸可分别在1、3、5分钟内分别实现3.07、2.78和4.97 log CFU / mL的对数降低。在氯气适应性细菌中,乙酰丙酸分别在1、3和5分钟内分别实现了2.77、3.07和5.21 log CFU / mL的对数降低。在热适应细菌上使用0.05%SDS和0.5%乙酰丙酸的混合物在1,3和5分钟内对数减少3.13、3.32和4.79 log CFU / mL,而在氯适应细菌上则达到3.20、3.33和5.66 log CFU / mL。接触时间的增加还增加了两种测试病原体的对数减少。长达72小时的存储时间导致两种测试病原体的对数减少。结果还表明,接触时间,储存时间和消毒剂之间存在显着差异(P≤0.05)。这项研究的结果可用于为新鲜农产品行业中适合热和氯的单核细胞增生李斯特氏菌选择合适的消毒剂和接触时间。

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