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首页> 美国卫生研究院文献>The ISME Journal >Survival of Desulfotomaculum spores from estuarine sediments after serial autoclaving and high-temperature exposure
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Survival of Desulfotomaculum spores from estuarine sediments after serial autoclaving and high-temperature exposure

机译：连续高压灭菌和高温暴露后河口沉积物中的脱硫母芽孢的存活

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Bacterial spores are widespread in marine sediments, including those of thermophilic, sulphate-reducing bacteria, which have a high minimum growth temperature making it unlikely that they grow in situ. These Desulfotomaculum spp. are thought to be from hot environments and are distributed by ocean currents. Their cells and spores upper temperature limit for survival is unknown, as is whether they can survive repeated high-temperature exposure that might occur in hydrothermal systems. This was investigated by incubating estuarine sediments significantly above (40–80 °C) maximum in situ temperatures (∼23 °C), and with and without prior triple autoclaving. Sulphate reduction occurred at 40–60 °C and at 60 °C was unaffected by autoclaving. Desulfotomaculum sp. C1A60 was isolated and was most closely related to the thermophilic D. kuznetsoviiT (∼96% 16S rRNA gene sequence identity). Cultures of Desulfotomaculum sp. C1A60, D. kuznetsoviiTand D. geothermicum B2T survived triple autoclaving while other related Desulfotomaculum spp. did not, although they did survive pasteurisation. Desulfotomaculum sp. C1A60 and D. kuznetsovii cultures also survived more extreme autoclaving (C1A60, 130 °C for 15 min; D. kuznetsovii, 135 °C for 15 min, maximum of 154 °C reached) and high-temperature conditions in an oil bath (C1A60, 130° for 30 min, D. kuznetsovii 140 °C for 15 min). Desulfotomaculum sp. C1A60 with either spores or predominantly vegetative cells demonstrated that surviving triple autoclaving was due to spores. Spores also had very high culturability compared with vegetative cells (∼30 × higher). Combined extreme temperature survival and high culturability of some thermophilic Desulfotomaculum spp. make them very effective colonisers of hot environments, which is consistent with their presence in subsurface geothermal waters and petroleum reservoirs.
机译：细菌孢子广泛存在于海洋沉积物中，包括嗜热，减少硫酸盐的细菌，其最低生长温度很高，因此不太可能原位生长。这些Desulfotomaculum spp。被认为是来自高温环境，并由洋流分配。它们的细胞和孢子存活的温度上限是未知的，因为它们是否可以在热液系统中可能发生的反复高温暴露下存活。通过将河口沉积物孵化到明显高于最高原位温度（〜23 C）（40–80 C）的条件，并在有或没有事先进行三重高压灭菌的情况下进行了研究。硫酸盐还原发生在40–60°C，并且在60°C时不受高压灭菌的影响。 Desulfotomaculum sp。分离出C1A60，它与嗜热的D. kuznetsovii T 密切相关（约96％的16S rRNA基因序列同一性）。 Desulfotomaculum sp。的文化。 C1A60，D。kuznetsovii T 和D. geothermicum B2T在三次高压灭菌后仍然存活，而其他相关的Desulfotomaculum spp。没有，尽管它们确实在巴氏灭菌法中存活了下来。 Desulfotomaculum sp。 C1A60和D. kuznetsovii培养物还可以在更极端的高压灭菌条件下存活（C1A60，130°C，15 min; D. kuznetsovii，135°C，15 min，最高154°C），并在油浴中保持高温条件（C1A60 ，130°30分钟，D。kuznetsovii 140°C 15分钟。 Desulfotomaculum sp。具有孢子或主要是营养细胞的C1A60证明存活的三次高压灭菌是由于孢子引起的。与营养细胞相比，孢子还具有很高的可培养性（约30倍以上）。结合了某些嗜热Desulfotomaculum spp的极端温度存活率和高可培养性。使它们成为高温环境下非常有效的定居者，这与它们在地下地热水和石油储层中的存在是一致的。

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