• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Marine Drugs >Oyster-Derived Zinc-Binding Peptide Modified by Plastein Reaction via Zinc Chelation Promotes the Intestinal Absorption of Zinc
【2h】

Oyster-Derived Zinc-Binding Peptide Modified by Plastein Reaction via Zinc Chelation Promotes the Intestinal Absorption of Zinc

机译：Plastein反应通过锌螯合修饰的牡蛎衍生锌结合肽可促进肠道对锌的吸收。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Zinc-binding peptides from oyster (Crassostrea gigas) have potential effects on zinc supplementation. The aim of this study was to prepare efficient zinc-binding peptides from oyster-modified hydrolysates by adding exogenous glutamate according to the plastein reaction and to further explore the zinc absorption mechanism of the peptide-zinc complex (MZ). The optimum conditions for the plastein reaction were as follows: pH 5.0, 40 °C, substrate concentration of 40%, pepsin dosage of 500 U/g, reaction time of 3 h and l-[1-13C]glutamate concentration of 10 mg/mL. The results of 13C isotope labelling suggested that the addition of l-[1-13C]glutamate contributed to the increase in the zinc-binding capacity of the peptide. The hydrophobic interaction was the main mechanism of action of the plastein reaction. Ultraviolet spectra and scanning electronic microscopy (SEM) revealed that the zinc-binding peptide could bind with zinc and form MZ. Furthermore, MZ could significantly enhance zinc bioavailability in the presence of phytic acid, compared to the commonly used ZnSO4. Additionally, MZ significantly promoted the intestinal absorption of zinc mainly through two pathways, the zinc ion channel and the small peptide transport pathway. Our work attempted to increase the understanding of the zinc absorption mechanism of MZ and to support the potential application of MZ as a supplementary medicine.
机译：牡蛎（Crassostrea gigas）的锌结合肽对补锌有潜在的影响。这项研究的目的是根据plastein反应，通过添加外源谷氨酸从牡蛎修饰的水解物中制备有效的锌结合肽，并进一步探索肽锌复合物（MZ）的锌吸收机理。 plastein反应的最佳条件为：pH 5.0、40°C，底物浓度为40％，胃蛋白酶剂量为500 U / g，反应时间为3 h和l- [1- 13 13 C同位素标记的结果表明，添加1- [1- 13 C]谷氨酸有助于增加该肽的锌结合能力。疏水相互作用是plastein反应的主要作用机理。紫外光谱和扫描电子显微镜（SEM）表明锌结合肽可以与锌结合形成MZ。此外，与常用的ZnSO4相比，MZ在存在植酸的情况下可以显着提高锌的生物利用度。此外，MZ主要通过两个途径（锌离子通道和小肽转运途径）显着促进锌的肠道吸收。我们的工作试图增进对MZ锌吸收机制的了解，并支持MZ作为补充药物的潜在应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号