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首页> 美国卫生研究院文献>Iranian Journal of Pharmaceutical Research : IJPR >TLC-Bioautography and GC-MS Analyses for Detection and Identification of Antioxidant Constituents of Trachyspermum copticum Essential Oil
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TLC-Bioautography and GC-MS Analyses for Detection and Identification of Antioxidant Constituents of Trachyspermum copticum Essential Oil

机译：TLC-Bioautography和GC-MS分析用于检测和鉴定黄连草精油中抗氧化剂成分

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摘要

The present work was designed to study the antioxidant activity and to identify the main active components of the essential oil of ajowan (Trachyspermum copticum) fruit. GC and GC-MS analyses of the essential oil showed the presence of eight compounds. The main constituents of the oil were thymol (43.7%), p-cymene (26.8%), and γ-terpinene (24.9%). The antioxidant and free radical scavenging activities of ajowan oil was evaluated by using ABTS•+ and β-carotene bleaching assays. The oil exhibited a considerable dose-dependent antioxidant activity. Antioxidant activity guided fractionation of the oil was carried out by TLC-bioautography method based on the DPPH• assay to screen and separate the main active constituents. The bioautography screening and fractionation resulted in the separation of the main antioxidant compound which was identified as thymol.
机译：本工作旨在研究抗氧化活性，并鉴定山楂（Trachyspermum copticum）水果精油的主要活性成分。精油的GC和GC-MS分析表明，存在八种化合物。油的主要成分是百里香酚（43.7％），对伞花烃（26.8％）和γ-萜品烯（24.9％）。采用ABTS •+ 和β-胡萝卜素漂白法评价了印度洋蓟油的抗氧化和清除自由基的活性。该油表现出相当大的剂量依赖性抗氧化活性。以TLPH-生物自显影法为基础，采用DPPH •法进行油的抗氧化活性指导分离，以筛选和分离出主要活性成分。生物自显影的筛选和分级分离导致了主要抗氧化剂化合物的分离，该化合物被确定为百里酚。

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