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首页> 美国卫生研究院文献>International Journal for Parasitology: Parasites and Wildlife >Detecting co-infections of Echinococcus multilocularis and Echinococcus canadensis in coyotes and red foxes in Alberta Canada using real-time PCR
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Detecting co-infections of Echinococcus multilocularis and Echinococcus canadensis in coyotes and red foxes in Alberta Canada using real-time PCR

机译：使用实时荧光定量PCR检测加拿大艾伯塔省土狼和赤狐中的多球棘球oc和加拿大棘球E的共感染

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The continued monitoring of Echinococcus species in intermediate and definitive hosts is essential to understand the eco-epidemiology of these parasites, as well to assess their potential impact on public health. In Canada, co-infections of Echinococcus canadensis and Echinococcus multilocularis based on genetic characterization have been recently reported in wolves, but not yet in other possible hosts such as coyotes and foxes. In this study, we aimed to develop a quantitative real-time PCR assay to detect E. multilocularis and E. canadensis and estimate the occurrence of co-infections while inferring about the relative abundance of the two parasites within hosts. We tested DNA extracted from aliquots of Echinococcus spp. specimens collected from intestinal tracts of 24 coyote and 16 fox carcasses from Alberta, Canada. We found evidence of co-infections of E. multilocularis and E. canadensis in 11 out of 40 (27%) samples, with 8 out of 24 (33%) in coyote samples and 3 out of 16 (19%) in red fox samples. DNA concentrations were estimated in three samples with Cq values within the range of the standard curve for both parasites; two of them presented higher DNA concentrations of E. multilocularis than E. canadensis. The use of qPCR aided detection of co-infections when morphological discrimination was difficult and quantification of DNA for samples within the standard curve. This is the first molecularly confirmed record of E. canadensis in coyotes and the first evidence of co-infections of E. multilocularis and E. canadensis in coyotes and red foxes.
机译：持续监测中型和定型寄主中的棘球species虫种类对于了解这些寄生虫的生态流行病学以及评估其对公共卫生的潜在影响至关重要。在加拿大，最近已经报道了在狼中发现了基于遗传特征的加拿大棘球oc和多眼棘球co的共感染，但在土狼和狐狸等其他可能的宿主中尚未见到这种感染。在这项研究中，我们旨在开发一种实时定量PCR检测法，以检测多叶大肠杆菌和加拿大E. canadensis，并估计共感染的发生，同时推断宿主体内两种寄生虫的相对丰度。我们测试了从棘球E虫等分试样中提取的DNA。从加拿大艾伯塔省的24只土狼和16只狐狸尸体的肠道收集标本。我们在40个样本中（27％）的11个中发现了多叶大肠杆菌和加拿大大肠埃希菌的共同感染的证据，土狼样本中24个（8％）的样本中有8个（16％）的红狐狸有3个样本（19％）的感染。样品。两种寄生虫的Cq值均在三个样品中，估计其DNA浓度；他们中的两个人提出的多叶E.DNA的浓度高于加拿大E. canadensis。当难以识别形态并在标准曲线内对样品进行DNA定量时，使用qPCR辅助检测共感染。这是土狼中加拿大大肠埃希菌的第一个分子确证记录，也是土狼和赤狐中多叶大肠杆菌和加拿大大肠埃希菌共感染的第一个证据。

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