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首页> 美国卫生研究院文献>International Journal of Nanomedicine >Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth
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Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

机译：咖啡油-海藻油基纳米乳剂的制备及其对UVA致小鼠皮肤损伤和黑色素瘤细胞生长的抑制作用研究

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Coffee grounds, a waste by-product generated after making coffee, contains approximately 15% coffee oil which can be used as a raw material in cosmetics. Algae oil rich in docosahexaenoic acid (DHA) has been demonstrated to possess anticancer and anti-inflammation functions. The objectives of this study were to develop a gas chromatography-mass spectrometry (GC-MS) method for the determination of fatty acids in coffee oil and algae oil and prepare a nanoemulsion for studying its inhibition effect on ultraviolet A-induced skin damage in mice and growth of melanoma cells B16-F10. A total of 8 and 5 fatty acids were separated and quantified in coffee oil and algae oil by GC-MS, respectively, with linoleic acid (39.8%) dominating in the former and DHA (33.9%) in the latter. A nanoemulsion with a particle size of 30 nm, zeta potential −72.72 mV, and DHA encapsulation efficiency 100% was prepared by using coffee oil, algae oil, surfactant (20% Span 80 and 80% Tween 80), and deionized water. Differential scanning calorimetry (DSC) analysis revealed a high stability of nanoemulsion when heated up to 110°C at a pH 6, whereas no significant changes in particle size distribution and pH occurred over a 90-day storage period at 4°C. Animal experiments showed that a dose of 0.1% coffee oil-algae oil nanoemulsion was effective in mitigating trans-epidermal water loss, skin erythema, melanin formation, and subcutaneous blood flow. Cytotoxicity test implied effective inhibition of melanoma cell growth by nanoemulsion with an IC50 value of 26.5 µg/mL and the cell cycle arrested at G2/M phase. A dose-dependent upregulation of p53, p21, cyclin B, and cyclin A expressions and downregulation of CDK1 and CDK2 occurred. Also, both Bax and cytochrome c expressions were upregulated and bcl-2 expression downregulated, accompanied by a rise in caspase-3, caspase-8, and caspase-9 activities for apoptosis execution. Collectively, the apoptosis pathway of melanoma cells B16-F10 may involve both mitochondria and death receptor.
机译：咖啡渣是煮咖啡后产生的废物副产物，其中含有约15％的咖啡油，可用作化妆品中的原料。富含二十二碳六烯酸（DHA）的藻类油已被证明具有抗癌和抗发炎的功能。这项研究的目的是开发一种气相色谱-质谱法（GC-MS）测定咖啡油和藻类油中的脂肪酸，并制备一种纳米乳剂，以研究其对紫外线A引起的小鼠皮肤损伤的抑制作用黑色素瘤细胞B16-F10的生长和生长。分别通过GC-MS在咖啡油和藻类油中分离和定量了8种和5种脂肪酸，其中亚油酸（39.8％）在DHA（33.9％）中占主导地位。通过使用咖啡油，藻油，表面活性剂（20％Span 80和80％Tween 80）和去离子水制备粒径为30 nm，ζ电势为-72.72 mV，DHA封装效率为100％的纳米乳液。差示扫描量热法（DSC）分析显示，在pH值为6的情况下加热至110°C时，纳米乳液具有很高的稳定性，而在4°C的90天存储期内，粒径分布和pH值均未发生显着变化。动物实验表明，0.1％的咖啡油-藻类油纳米乳剂可有效缓解表皮水分流失，皮肤红斑，黑色素形成和皮下血流。细胞毒性试验表明，通过纳米乳剂可有效抑制黑色素瘤细胞的生长，IC50值为26.5 µg / mL，细胞周期停滞在G2 / M期。发生了剂量依赖性的p53，p21，cyclin B和cyclin A表达上调以及CDK1和CDK2的下调。同样，Bax和细胞色素c的表达均被上调，而bcl-2的表达均被下调，伴随着caspase-3，caspase-8和caspase-9活性的增加，导致细胞凋亡。总的来说，黑色素瘤细胞B16-F10的凋亡途径可能涉及线粒体和死亡受体。

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