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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Genetic Diversity and Phylogeny of Antagonistic Bacteria against Phytophthora nicotianae Isolated from Tobacco Rhizosphere
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Genetic Diversity and Phylogeny of Antagonistic Bacteria against Phytophthora nicotianae Isolated from Tobacco Rhizosphere

机译：烟草根际分离株对烟草疫霉的拮抗细菌的遗传多样性和系统发育

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摘要

The genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by BOXAIR-PCR, 16S-RFLP, 16S rRNA sequence homology and phylogenetic analysis methods. These studies revealed that 4.01% of the 6652 tested had some inhibitory activity against Phytophthora nicotianae. BOXAIR-PCR analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. A total of 25 16S-RFLP patterns were identified representing over 33 species from 17 different genera. Our results also found a significant amount of bacterial diversity among the antagonistic bacteria compared to other published reports. For the first time; Delftia tsuruhatensis, Stenotrophomonas maltophilia, Advenella incenata, Bacillus altitudinis, Kocuria palustris, Bacillus licheniformis, Agrobacterium tumefaciens and Myroides odoratimimus are reported to display antagonistic activity towards Phytophthora nicotianae. Furthermore, the majority (75%) of the isolates assayed for antagonistic activity were Gram-positives compared to only 25% that were Gram-negative bacteria.
机译：通过BOXAIR-PCR，16S-RFLP，16S rRNA序列同源性和系统发育分析方法检查了烟草根际拮抗细菌的遗传多样性。这些研究表明，所测试的6652中有4.01％具有对烟草疫霉菌的抑制活性。 BOXAIR-PCR分析显示35种不同的扩增子以91％的相似性水平排列，反映了拮抗细菌之间高度的基因型多样性。共鉴定出25种16S-RFLP模式，分别代表来自17个不同属的33种。与其他已发表的报道相比，我们的结果还发现拮抗细菌之间存在大量细菌多样性。首次;据报道，德尔福tsuruhatensis，嗜麦芽窄食单胞菌，小叶阿德温氏杆菌，枯草芽孢杆菌，古柯菌，地衣芽孢杆菌，根癌农杆菌和香豆Myroides对烟草疫霉菌显示出拮抗活性。此外，分析出具有拮抗活性的分离株中的大多数（75％）是革兰氏阳性菌，而革兰氏阴性菌只有25％。

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