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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Protein Structures among Bio-Ethanol Co-Products and Its Relationships with Ruminal and Intestinal Availability of Protein in Dairy Cattle
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Protein Structures among Bio-Ethanol Co-Products and Its Relationships with Ruminal and Intestinal Availability of Protein in Dairy Cattle

机译：生物乙醇副产物中的蛋白质结构及其与奶牛蛋白质的瘤胃和肠道可利用性的关系

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摘要

The objectives of this study were to reveal molecular structures of protein among different types of the dried distillers grains with solubles (100% wheat DDGS (WDDGS); DDGS blend1 (BDDGS1, corn to wheat ratio 30:70%); DDGS blend2 (BDDGS2, corn to wheat ratio 50:50 percent)) and different batches within DDGS type using diffuse reflectance infrared Fourier transform spectroscopy (DRIFT). Compared with BDDGS1 and BDDGS2, wheat DDGS had higher (p < 0.05) peak area intensities of protein amide I and II and amide I to II intensity ratio. Increasing the corn to wheat ratio form 30:70 to 50:50 in the blend DDGS did not affect amide I and II area intensities and their ratio. Amide I to II peak intensity ratio differed (p < 0.05) among the different batches within WDDGS and BDDGS1. Compared with both blend DDGS types, WDDGS had higher α-helix and β-sheet ratio (p < 0.05), while α-helix to β-sheet ratio was similar among the three DDGS types. The α-helix to β-sheet ratio differed significantly among batches within WDDGS. Principal component analysis (PCA) revealed that protein molecular structures in WDDGS differed from those of BDDGS1 and between different batches within BDDGS1 and BDDGS2. The α-helix to β-sheet ratios of protein in all DDGS types had an influence on availability of protein at the ruminal level as well as at the intestinal level. The α-helix to β-sheet ratio was positively correlated to rumen undegraded protein (r = 0.41, p < 0.05) and unavailable protein (PC; r = 0.59, p < 0.05).
机译：这项研究的目的是揭示具有可溶物（100％小麦DDGS（WDDGS）； DDGS blend1（BDDGS1，玉米与小麦的比例为30:70％）； DDGS blend2（BDDGS2））的不同类型的干酒糟中蛋白质的分子结构。 ，玉米与小麦的比例为50：50％）和使用漫反射红外傅里叶变换光谱（DRIFT）的DDGS类型中的不同批次。与BDDGS1和BDDGS2相比，小麦DDGS具有更高的（p <0.05）峰值峰强度的蛋白质酰胺I和II和酰胺I与II强度比。在掺混物DDGS中将玉米与小麦的比例从30:70增加到50:50不会影响酰胺I和II的面积强度及其比例。 WDDGS和BDDGS1内不同批次的酰胺I与II峰强度之比有所不同（p <0.05）。与两种混合DDGS类型相比，WDDGS具有更高的α螺旋和β折叠比率（p <0.05），而三种DDGS中α螺旋与β折叠比率相似。 WDDGS中的批次之间，α螺旋与β折叠的比率存在显着差异。主成分分析（PCA）显示WDDGS中的蛋白质分子结构与BDDGS1的蛋白质分子结构不同，并且在BDDGS1和BDDGS2的不同批次之间。在所有DDGS类型中，蛋白质的α-螺旋与β-片层之比对瘤胃和肠内蛋白质的利用率都有影响。 α-螺旋与β-折叠的比例与瘤胃未降解蛋白（r = 0.41，p <0.05）和不可用蛋白（PC； r = 0.59，p <0.05）呈正相关。

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