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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Mitochondrial Cytochrome c Oxidase and F1Fo-ATPase Dysfunction in Peppers (Capsicum annuum L.) with Cytoplasmic Male Sterility and Its Association with orf507 and Ψatp6-2 Genes
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Mitochondrial Cytochrome c Oxidase and F1Fo-ATPase Dysfunction in Peppers (Capsicum annuum L.) with Cytoplasmic Male Sterility and Its Association with orf507 and Ψatp6-2 Genes

机译：具有细胞质雄性不育辣椒的辣椒线粒体细胞色素c氧化酶和F1Fo-ATPase功能异常及其与orf507和Ψatp6-2基因的关系

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Cytoplasmic male sterility (CMS) in pepper (Capsicum annuum L.) has been associated with novel genes in the mitochondria, such as orf507 and Ψatp6-2. Plant sterility has been proved to result from the rearrangement of the mitochondrial genome. Previous studies have demonstrated that orf507 is co-transcribed with the cox II gene, and Ψatp6-2 is truncated at the 3′ region of the atp6-2 that is found in the maintainer line. Until this time, little has been known about the relationship between the novel gene and the function of its corresponding enzyme in mitochondria from the CMS pepper line. Moreover, the aberrant function of the mitochondrial enzymes is seldom reported in pepper. In this study, we observed that anther abortion occurred after the tetrad stage in the CMS line (HW203A), which was accompanied by premature programmed cell death (PCD) in the tapetum. The spatiotemporal expression patterns of orf507 and Ψatp6-2 were analyzed together with the corresponding enzyme activities to investigate the interactions of the genes and mitochondrial enzymes. The two genes were both highly expressed in the anther. The orf507 was down-regulated in HW203A (CMS line), with nearly no expression in HW203B (the maintainer line). In contrast, the cytochrome c oxidase activity in HW203A showed the opposite trend, reaching its highest peak at the tetrad stage when compared with HW203B at the same stage. The Ψatp6-2 in the CMS line was also down-regulated, but it was up-regulated in the maintainer line. The corresponding F1Fo-ATPase activity in the CMS line was gradually decreased along with the development of the anther, which showed the same trend for Ψatp6-2 gene expression. On the contrary, with up-regulated gene expression of atp6-2 in the maintainer line, the F1Fo-ATPase activity sharply decreased after the initial development stage, but gradually increased following the tetrad stage, which was contrary to what happened in the CMS line. Taken together, all these results may provide evidence for the involvement of aberrant mitochondrial cytochrome c oxidase and F1Fo-ATPase in CMS pepper anther abortion. Moreover, the novel orf507 and Ψatp6-2 genes in the mitochondria may be involved in the dysfunction of the cytochrome c oxidase and F1Fo-ATPase, respectively, which are responsible for the abortion of anthers in the CMS line.
机译：辣椒（Capsicum annuum L.）的细胞质雄性不育（CMS）与线粒体中的新基因，例如orf507和Ψatp6-2相关。已证明植物不育是由线粒体基因组的重排导致的。先前的研究表明，orf507与cox II基因共转录，并且在维持系中atp6-2的3'区截短了Ψatp6-2。直到现在，从CMS辣椒系了解到的新基因与其线粒体中相应酶的功能之间的关系还鲜为人知。此外，辣椒中很少报道线粒体酶的异常功能。在这项研究中，我们观察到花药流产发生在CMS系（HW203A）的第四个阶段之后，并伴随着绒毡层中过早的程序性细胞死亡（PCD）。分析了orf507和Ψatp6-2的时空表达模式以及相应的酶活性，以研究基因与线粒体酶的相互作用。这两个基因都在花药中高表达。 orf507在HW203A（CMS行）中下调，而在HW203B（维持线）中几乎没有表达。相反，HW203A中的细胞色素C氧化酶活性显示相反的趋势，与同一阶段的HW203B相比，在四联体阶段达到最高峰。 CMS系中的Ψatp6-2也下调，但在维护系中上调。随着花药的发育，CMS系中相应的F1Fo-ATPase活性逐渐降低，这与Ψatp6-2基因的表达趋势相同。相反，随着维持系中atp6-2基因表达的上调，F1Fo-ATPase活性在初始发育阶段后急剧下降，而在第四个阶段后逐渐上升，这与CMS系发生的情况相反。综上所述，所有这些结果可能为线粒体细胞色素c氧化酶和F1Fo-ATPase异常参与CMS花药流产提供证据。此外，线粒体中新的orf507和Ψatp6-2基因可能分别参与细胞色素c氧化酶和F1Fo-ATPase的功能障碍，这是造成CMS系花药流产的原因。

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