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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Lactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Possible Role in Protection against Oxidative DNA Damage
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Lactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Possible Role in Protection against Oxidative DNA Damage

机译：乳铁蛋白直接清除羟基自由基并进行氧化自降解：可能对氧化DNA损伤的保护作用。

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In this study, we examined the protective effect of lactoferrin against DNA damage induced by various hydroxyl radical generation systems. Lactoferrin (LF) was examined with regard to its potential role as a scavenger against radical oxygen species using bovine milk LF. Native LF, iron-saturated LF (holo-LF), and apolactoferrin (apo-LF) effectively suppressed strand breaks in plasmid DNA due to hydroxyl radicals produced by the Fenton reaction. In addition, both native LF and holo-LF clearly protected calf thymus DNA from fragmentation due to ultraviolet irradiation in the presence of H2O2. We also demonstrated a protective effect of all three LF molecules against 8-hydroxydeoxyguanosine (8-OHdG) formation in calf thymus DNA following ultraviolet (UV) irradiation with H2O2. Our results clearly indicate that native LF has reactive oxygen species-scavenging ability, independent of its nature as a masking component for transient metals. We also demonstrated that the protective effect of LF against oxidative DNA damage is due to degradation of LF itself, which is more susceptible to degradation than other bovine milk proteins.
机译：在这项研究中，我们检查了乳铁蛋白对各种羟基自由基产生系统引起的DNA损伤的保护作用。使用牛乳LF检查了乳铁蛋白（LF）作为清除自由基氧的清除剂的潜在作用。天然LF，铁饱和LF（holo-LF）和apolactoferrin（apo-LF）有效抑制质粒DNA中由于Fenton反应产生的羟基而引起的链断裂。此外，天然LF和全氟LF都清楚地保护了小牛胸腺DNA免受H2O2存在下的紫外线照射造成的碎裂。我们还证明了在紫外线（UV）照射H2O2后，所有三个LF分子对小牛胸腺DNA中的8-羟基脱氧鸟苷（8-OHdG）形成的保护作用。我们的结果清楚地表明，天然LF具有清除活性氧的能力，与其作为瞬态金属的掩蔽组分的性质无关。我们还证明了LF抗氧化DNA损伤的保护作用是由于LF本身的降解所致，它比其他牛乳蛋白更易降解。

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