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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >A PPO Promoter from Betalain-Producing Red Swiss Chard Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants
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A PPO Promoter from Betalain-Producing Red Swiss Chard Directs Petiole- and Root-Preferential Expression of Foreign Gene in Anthocyanins-Producing Plants

机译：来自生产甜菜碱的红色瑞士甜菜的PPO启动子指导花青素生产植物中外源基因的叶柄和根优先表达。

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摘要

A 1670 bp 5′-flanking region of the polyphenol oxidase (PPO) gene was isolated from red Swiss chard, a betalain-producing plant. This region, named promoter BvcPPOP, and its 5′-truncated versions were fused with the GUS gene and introduced into Arabidopsis, an anthocyanins-producing plant. GUS histochemical staining and quantitative analysis of transgenic plants at the vegetative and reproductive stages showed that BvcPPOP could direct GUS gene expression in vegetative organs with root- and petiole-preference, but not in reproductive organs including inflorescences shoot, inflorescences leaf, flower, pod and seed. This promoter was regulated by developmental stages in its driving strength, but not in expression pattern. It was also regulated by the abiotic stressors tested, positively by salicylic acid (SA) and methyl jasmonate (MeJA) but negatively by abscisic acid (ABA), gibberellin (GA), NaCl and OH−. Its four 5′-truncated versions varied in the driving strength, but not obviously in expression pattern, and even the shortest version (−225 to +22) retained the root- and petiole- preference. This promoter is, to our knowledge, the first PPO promoter cloned and functionally elucidated from the betalain-producing plant, and thus provides not only a useful tool for expressing gene(s) of agricultural interest in vegetative organs, but also a clue to clarify the function of metabolism-specific PPO in betalain biosynthesis.
机译：从产甜菜碱的红色瑞士甜菜中分离出多酚氧化酶（PPO）基因的1670 bp 5'侧翼区域。将该区域称为启动子BvcPPOP，并将其5'截短版本与GUS基因融合，并引入到生产花青素的植物拟南芥中。 GUS组织化学染色和转基因植物在营养和生殖阶段的定量分析表明，BvcPPOP可以指导GUS基因在根和叶柄优先的营养器官中表达，但不能在生殖器官（包括花序芽，花序叶，花，荚果和种子。该启动子在其驱动强度上受发育阶段的调节，但不受表达模式的调节。它也受测试的非生物胁迫的调节，水杨酸（SA）和茉莉酸甲酯（MeJA）呈阳性，而脱落酸（ABA），赤霉素（GA），NaCl和OH -呈负相关。它的四个5'截短版本在驱动强度上各不相同，但在表达方式上没有明显变化，甚至最短的版本（-225至+22）也保留了根和叶柄的优先选择。据我们所知，该启动子是从产甜菜碱的植物中克隆并功能性阐明的第一个PPO启动子，因此不仅为在营养器官中表达农业感兴趣的基因提供了有用的工具，而且为阐明这一点提供了线索。代谢特异性PPO在甜菜碱生物合成中的功能

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