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首页> 美国卫生研究院文献>Dong wu xue yan jiu = Zoological Research >Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains
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Comparative study of the transfection efficiency of commonly used viral vectors in rhesus monkey (Macaca mulatta) brains

机译：恒河猴（Macaca mulatta）大脑中常用病毒载体转染效率的比较研究

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摘要

Viral vector transfection systems are among the simplest of biological agents with the ability to transfer genes into the central nervous system. In brain research, a series of powerful and novel gene editing technologies are based on these systems. Although many viral vectors are used in rodents, their full application has been limited in non-human primates. To identify viral vectors that can stably and effectively express exogenous genes within non-human primates, eleven commonly used recombinant adeno-associated viral and lentiviral vectors, each carrying a gene to express green or red fluorescence, were injected into the parietal cortex of four rhesus monkeys. The expression of fluorescent cells was used to quantify transfection efficiency. Histological results revealed that recombinant adeno-associated viral vectors, especially the serotype 2/9 coupled with the cytomegalovirus, human synapsin Ⅰ, or Ca2+/calmodulin-dependent protein kinase Ⅱ promoters, and lentiviral vector coupled with the human ubiquitin C promoter, induced higher expression of fluorescent cells, representing high transfection efficiency. This is the first comparison of transfection efficiencies of different viral vectors carrying different promoters and serotypes in non-human primates (NHPs). These results can be used as an aid to select optimal vectors to transfer exogenous genes into the central nervous system of non-human primates.
机译：病毒载体转染系统是最简单的生物制剂之一，具有将基因转移到中枢神经系统的能力。在大脑研究中，基于这些系统的一系列强大而新颖的基因编辑技术。尽管啮齿动物中使用了许多病毒载体，但它们在非人类灵长类动物中的完整应用受到了限制。为了鉴定能够稳定和有效表达非人类灵长类动物中外源基因的病毒载体，将十一种常用的重组腺相关病毒和慢病毒载体分别注入一种表达绿色或红色荧光的基因，并注入到四只恒河猴的顶叶皮层中。猴子。荧光细胞的表达用于定量转染效率。组织学结果表明，重组腺相关病毒载体，特别是血清型2/9，与巨细胞病毒，人突触蛋白Ⅰ或Ca 2 + /钙调蛋白依赖性蛋白激酶Ⅱ启动子偶联，以及慢病毒载体偶联用人泛素C启动子诱导荧光细胞的更高表达，代表高转染效率。这是在非人灵长类动物（NHP）中携带不同启动子和血清型的不同病毒载体转染效率的首次比较。这些结果可以用作选择最佳载体以将外源基因转移到非人类灵长类动物的中枢神经系统的辅助手段。

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