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首页> 美国卫生研究院文献>Frontiers in Microbiology >Occurrence and Antibiotic Resistance of Vibrio parahaemolyticus from Shellfish in Selangor, Malaysia
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Occurrence and Antibiotic Resistance of Vibrio parahaemolyticus from Shellfish in Selangor, Malaysia

机译：马来西亚雪兰莪州贝类副溶血性弧菌的发生和耐药性

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High consumer demand for shellfish has led to the need for large-scale, reliable shellfish supply through aquaculture or shellfish farming. However, bacterial infections which can spread rapidly among shellfish poses a major threat to this industry. Shellfish farmers therefore often resort to extensive use of antibiotics, both prophylactically and therapeutically, in order to protect their stocks. The extensive use of antibiotics in aquaculture has been postulated to represent a major contributing factor in the rising incidence of antimicrobial resistant pathogenic bacteria in shellfish. This study aimed to investigate the incidence of pathogenic Vibrio parahaemolyticus and determine the antibiotic resistance profile as well as to perform plasmid curing in order to determine the antibiotic resistance mediation. Based on colony morphology, all 450 samples tested were positive for Vibrio sp; however, tox-R assay showed that only 44.4% (200/450) of these were V. parahaemolyticus. Out of these 200 samples, 6.5% (13/200) were trh-positive while none were tdh-positive. Antibiotic resistance was determined for all V. parahaemolyticus identified against 14 commonly used antibiotics and the multiple antibiotic resistance index (MAR) was calculated. The isolates demonstrated high resistance to several antibiotics tested- including second and third-line antibiotics- with 88% resistant to ampicillin, 81% to amikacin,70.5% to kanamycin, 73% to cefotaxime, and 51.5% to ceftazidime. The MAR index ranged from 0.00 to 0.79 with the majority of samples having an index of 0.36 (resistant to five antibiotics). Among the 13 trh-positive strains, almost 70% (9/13) demonstrated resistance to 4 or more antibiotics. Plasmid profiling for all V. parahaemolyticus isolates revealed that 86.5% (173/200) contained plasmids - ranging from 1 to 7 plasmids with DNA band sizes ranging from 1.2 kb to greater than 10 kb. 6/13 of the pathogenic V. pathogenic strains contained plasmid. After plasmid curing, the plasmid containing pathogenic strains isolated in our study have chromosomally mediated ampicillin resistance while the remaining resistance phenotypes are plasmid mediated. Overall, our results indicate that while the incidence of pathogenic V. parahaemolyticus in shellfish in Selangor still appears to be at relatively reassuring levels, antibiotic resistance is a real concern and warrants ongoing surveillance.
机译：消费者对贝类的需求旺盛，因此需要通过水产养殖或贝类养殖来大规模，可靠地供应贝类。但是，细菌感染会迅速在贝类中传播，这对该行业构成了重大威胁。因此，贝类养殖者经常在预防和治疗上广泛使用抗生素，以保护其种群。据推测，抗生素在水产养殖中的广泛使用代表了贝类中抗菌素耐药病原菌发病率上升的主要因素。这项研究旨在调查致病性副溶血性弧菌的发生率并确定抗生素耐药性，并进行质粒固化以测定抗生素耐药性。根据菌落形态，测试的所有450个样品的弧菌属均为阳性。但是，tox-R分析显示，其中只有44.4％（200/450）是副溶血性弧菌。在这200个样本中，有6.5％（13/200）是trh阳性的，而没有tdh阳性的。确定所有针对14种常用抗生素的副溶血性弧菌的抗生素耐药性，并计算多重抗生素耐药性指数（MAR）。分离株对几种抗生素（包括二线和三线抗生素）表现出高抗性，其中对氨苄西林的抗药性为88％，对阿米卡星的抗药性为81％，对卡那霉素的抗性为70.5％，对头孢噻肟的抗性为73％，对头孢他啶的抗性为51.5％。 MAR指数的范围从0.00到0.79，大多数样品的指数为0.36（对五种抗生素有抗性）。在13种trh阳性菌株中，几乎70％（9/13）表现出对4种或更多种抗生素的抗性。对所有副溶血性弧菌分离株的质粒分析表明，有86.5％（173/200）的质粒-1至7个质粒，DNA条带大小为1.2 kb至大于10 kb。 6/13的致病性V.致病菌株含有质粒。质粒固化后，在我们的研究中分离出的含有病原菌株的质粒具有染色体介导的氨苄青霉素抗性，而其余的耐药表型则由质粒介导。总的来说，我们的结果表明，尽管在雪兰莪州的贝类中，致病性副溶血性弧菌的发生率仍处于相对令人放心的水平，但抗生素耐药性仍是一个真正的问题，需要进行持续监测。

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