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首页> 美国卫生研究院文献>Genetics and Molecular Biology >Genotoxic evaluation of the River Paranaíba hydrographic basin in MonteCarmelo, MG, Brazil, by the Tradescantiamicronucleus
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Genotoxic evaluation of the River Paranaíba hydrographic basin in MonteCarmelo, MG, Brazil, by the Tradescantiamicronucleus

机译：蒙特州巴拉那伊巴河水文盆地的遗传毒性评价巴西MG的卡梅洛，通过Tradescantia微核

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摘要

Pollutants have adverse effects on human health and on other organisms that inhabit or use water resources. The aim of the present study was to assess the environmental quality of three watercourses in Monte Carmelo, MG, Brazil, using the micronucleus test on Tradescantia. For each treatment, 15 plants were exposed to water samples for 24 h. The control group was exposed to formaldehyde (0.2%) and the negative control to Hoagland solution. Subsequently the plants were placed in Hoagland solution for 24 h to recover. Cells were stained with 2% acetic carmine and examined by light microscopy. Three hundred tetrads were analyzed per slide. The frequency of genotoxic alterations was expressed as the number of micronuclei per 100 tetrads, and the groups were compared by ANOVA. At all sample sites for each watercourse significant genotoxicity indices were observed. The results suggest that in the Mumbuca creek, the current situation of effluent discharge should be reconsidered by the municipal environmental authorities. The increase in micronucleus frequency denoted for water samples of the Mumbuca creek, Lambari river and Perdizes river emphasizes the need to adopt environmental vigilance strategies, such as biological monitoring.
机译：污染物会对人类健康和其他居住或使用水资源的生物造成不利影响。本研究的目的是使用对Trade虫的微核试验来评估巴西MG蒙特卡梅洛的三个水道的环境质量。对于每次处理，将15株植物暴露于水样品中24小时。对照组暴露于甲醛（0.2％），阴性对照组暴露于Hoagland溶液。随后将植物置于Hoagland溶液中24小时以恢复。细胞用2％乙酸胭脂红染色并通过光学显微镜检查。每个载玻片分析了300个四联体。遗传毒性改变的频率表示为每100个四分位数的微核数，并通过ANOVA比较各组。在每个水道的所有样本位置，均观察到了显着的遗传毒性指数。结果表明，在孟买卡河，污水排放的现状应由市环保部门重新考虑。孟买卡河，兰巴里河和Perdizes河的水样中所显示的微核频率增加，强调需要采取环境警惕性策略，例如生物监测。

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