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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation
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Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

机译：透明质酸/骨替代复合物植入鸡胚绒毛尿囊膜可诱导成骨细胞分化和血管生成，但不引起炎症

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Microscopic and molecular events related to alveolar ridge augmentation are less known because of the lack of experimental models and limited molecular markers used to evaluate this process. We propose here the chick embryo chorioallantoic membrane (CAM) as an in vivo model to study the interaction between CAM and bone substitutes (B) combined with hyaluronic acid (BH), saline solution (BHS and BS, respectively), or both, aiming to point out the microscopic and molecular events assessed by Runt-related transcription factor 2 (RUNX 2), osteonectin (SPARC), and Bone Morphogenic Protein 4 (BMP4). The BH complex induced osteoprogenitor and osteoblastic differentiation of CAM mesenchymal cells, certified by the RUNX2 +, BMP4 +, and SPARC + phenotypes capable of bone matrix synthesis and mineralization. A strong angiogenic response without inflammation was detected on microscopic specimens of the BH combination compared with an inflammatory induced angiogenesis for the BS and BHS combinations. A multilayered organization of the BH complex grafted on CAM was detected with a differential expression of RUNX2, BMP4, and SPARC. The BH complex induced CAM mesenchymal cells differentiation through osteoblastic lineage with a sustained angiogenic response not related with inflammation. Thus, bone granules resuspended in hyaluronic acid seem to be the best combination for a proper non-inflammatory response in alveolar ridge augmentation. The CAM model allows us to assess the early events of the bone substitutes–mesenchymal cells interaction related to osteoblastic differentiation, an important step in alveolar ridge augmentation.
机译：由于缺乏实验模型和用于评估该过程的有限分子标记，与肺泡增大相关的微观和分子事件鲜为人知。我们在这里提出雏鸡胚胎尿囊膜（CAM）作为体内模型，以研究CAM与骨替代物（B）结合透明质酸（BH），盐溶液（分别为BHS和BS）或两者的相互作用。指出由Runt相关转录因子2（RUNX 2），骨连接蛋白（SPARC）和骨形态发生蛋白4（BMP4）评估的微观和分子事件。 BH复合物诱导CAM间充质细胞的骨祖细胞和成骨细胞分化，并通过能够进行骨基质合成和矿化的RUNX2 +，BMP4 +和SPARC +表型进行验证。与BS和BHS组合的炎症诱导的血管生成相比，在BH组合的显微镜标本上检测到了强烈的无炎症的血管生成反应。检测到嫁接于CAM的BH复合物的多层组织，其RUNX2，BMP4和SPARC的差异表达。 BH复合物通过成骨细胞谱系诱导CAM间充质细胞分化，并具有与炎症无关的持续性血管生成反应。因此，悬浮在透明质酸中的骨颗粒似乎是在牙槽增大中适当的非炎性反应的最佳组合。 CAM模型使我们能够评估与成骨细胞分化有关的骨替代物-间充质细胞相互作用的早期事件，这是牙槽隆起的重要步骤。

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