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首页> 美国卫生研究院文献>Journal of Anatomy and Physiology >The innervation of the splenic capsule in the guinea pig: an immunohistochemical and ultrastructural study.
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The innervation of the splenic capsule in the guinea pig: an immunohistochemical and ultrastructural study.

机译：豚鼠脾脏胶囊的神经支配：一项免疫组织化学和超微结构研究。

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The innervation of the capsule of the guinea pig spleen was studied by light microscopy using an indirect fluorescent-labelled antibody technique, as well as by electron microscopy. A dense network of nerve fibres immunoreactive to the general neuronal marker, protein gene product 9.5 was observed in tangential sections through the capsule corresponding to the subcapsular compartment. The PGP 9.5-immunoreactivity in the fibres appeared to a large extent to be colocalised with tyrosine hydroxylase and neuropeptide Y (NPY) immunoreactivities as well as with synaptophysin immunoreactivity. Only very occasional fibres with substance P or calcitonin-gene-related peptide immunoreactivity were observed in tangential sections of the capsular region. By electron microscopy unmyelinated nerve fibres in the capsule were found to contain a large number of small dense-cored as well as clear vesicles and large dense-cored vesicles in varicose parts of the axons. The axolemma of the varicose regions was often naked, devoid of Schwann cells, and sometimes appeared denser than the nonspecialised parts of the membrane. These naked regions were observed in single sections to be apposed to splenic cells with variable intervals of extracellular space and interposed basal lamina material. Another type of contact was characterised by a very close association with splenic cells with no basal lamina interposed between the plasma membranes of the axon and the splenic cell. An intimate ultrastructural relationship was often also seen between varicose vesicle-containing axons and neighbouring axons in the nerve fibre bundles. The results show that the splenic capsule and its immediate neighbouring regions are innervated by catecholaminergic, NPY-containing fibres, which appear to establish different types of relations with the splenic cells as well as with one another.
机译：通过使用间接荧光标记抗体技术的光学显微镜以及电子显微镜研究豚鼠脾脏胶囊的神经支配。在与囊下部分相对应的穿过囊的切线部分中观察到了与一般神经元标记蛋白基因产物9.5有免疫反应性的密集神经纤维网络。纤维中的PGP 9.5免疫反应性在很大程度上与酪氨酸羟化酶和神经肽Y（NPY）免疫反应性以及突触素免疫反应性共定位。在囊区域的切向部分仅观察到极少数具有P物质或降钙素基因相关肽免疫反应性的纤维。通过电子显微镜检查，发现胶囊中无髓鞘的神经纤维在轴突的静脉曲张部分含有大量的小致密的和透明的囊泡以及大的致密的囊泡。静脉曲张区域的腋窝通常是裸露的，没有雪旺细胞，有时看起来比膜的非专门部分更密。在单个切片中观察到这些裸露区域与脾细胞并存，这些脾细胞具有可变间隔的细胞外空间和插入的基底层材料。另一类接触的特征是与脾细胞紧密结合，在轴突和脾细胞的质膜之间没有基底层。在含曲张小泡的轴突和神经纤维束中邻近的轴突之间也经常发现密切的超微结构关系。结果表明，脾囊及其附近区域受儿茶酚胺能，含NPY的纤维的支配，它们似乎与脾细胞以及彼此之间建立了不同类型的关系。

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