首页> 美国卫生研究院文献>Journal of Anatomy >Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra)
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Characterisation of glycoconjugate sugar residues in the vomeronasal organ of the armadillo Chaetophractus villosus (Mammalia, xenarthra)

机译:犰狳Chaetophractus villosus(哺乳动物,xenarthra)的犁鼻鼻器官中的糖缀合物糖残基的表征

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摘要

Conventional carbohydrate histochemistry and the binding patterns of 21 lectins were analysed to characterise the glycoconjugate content in the components of the vomeronasal organ of the armadillo Chaetophractus villosus. The mucomicrovillous complex of the sensory epithelium bound most of the lectins studied. No reaction was observed with Con A, PSA, S-Con A and SBA, and the sustentacular cells were stained with UEA-I, DSL, LEL, STL and Con A. The vomeronasal receptor neurons were labelled with S-WGA, WGA, PNA, UEA-I, STL, Con A, S-Con A, ECL and RCA120. The basal cell layer reacted with S-WGA, WGA, LCA, UEA-I, DSL, LEL, STL, Con A, JAC and VVA. The nonsensory epithelium exhibited a differential staining in relation to the different components. The mucociliary complex stained with ECL, DBA, JAC, RCA120, STL, LCA, PHA-E, PHA-L, LEL, BSL-I and VVA. However, SJA and UEA-I stained the mucus complex lining a subpopulation of columnar cells. The cytoplasm and cell membranes of columnar cells was labelled with DBA, DSL and LCA. The apical region of these cells exhibited moderate reactivity with LEL and SJA. None of the lectins bound specifically to secretory granules of the nonsecretory cells. Basal cells of the nonsensory epithelium were labelled with DSL, LEL, LCA, BSL-I and STL. The vomeronasal glands showed a positive reaction with WGA, DSL, LEL, LCA, DBA, PNA, RCA120 and SBA. Subpopulations of acinar cells were observed with ECL, S-WGA, Con A, S-Con A and DBA. PNA and RCA120 stained the cells lining the glandular ducts. In comparison with previous results obtained in the olfactory mucosa of the same group of armadillos, the carbohydrate composition of the vomeronasal organ sensory epithelium differed from the olfactory sensory epithelium. This is probably related to the different nature of molecules involved in the perireceptor processes.
机译:分析了常规碳水化合物的组织化学和21种凝集素的结合模式,以表征犰狳Chaetophractus villosus的犁鼻器器官各成分中的糖缀合物含量。感觉上皮的粘膜-静脉复合物结合了大多数研究的凝集素。 Con A,PSA,S-Con A和SBA均未观察到反应,并且Sueentacular细胞用UEA-1,DSL,LEL,STL和Con A染色。犁鼻器受体神经元标记有S-WGA,WGA, PNA,UEA-1,STL,Con A,S-Con A,ECL和RCA120。基底细胞层与S-WGA,WGA,LCA,UEA-1,DSL,LEL,STL,Con A,JAC和VVA反应。非感觉上皮相对于不同组分表现出差异染色。粘膜纤毛复合物用ECL,DBA,JAC,RCA120,STL,LCA,PHA-E,PHA-L,LEL,BSL-1和VVA染色。但是,SJA和UEA-I将粘液复合物染色成一列柱状细胞亚群。柱状细胞的细胞质和细胞膜分别用DBA,DSL和LCA标记。这些细胞的顶端区域表现出与LEL和SJA的中等反应性。没有一种凝集素特异性结合非分泌细胞的分泌颗粒。非感觉上皮的基底细胞用DSL,LEL,LCA,BSL-1和STL标记。犁鼻腺与WGA,DSL,LEL,LCA,DBA,PNA,RCA120和SBA呈阳性反应。用ECL,S-WGA,Con A,S-Con A和DBA观察到腺泡细胞亚群。 PNA和RCA120染色了位于腺管内的细胞。与以前在同一组犰狳的嗅觉粘膜中获得的结果相比,犁鼻鼻器官感觉上皮的碳水化合物组成与嗅觉感觉上皮不同。这可能与周围感受器过程中涉及的分子的不同性质有关。

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