BackgroundLinear regression of efficiency (LRE) introduced a new paradigm for real-time qPCR that enables large-scale absolute quantification by eliminating the need for standard curves. Developed through the application of sigmoidal mathematics to SYBR Green I-based assays, target quantity is derived directly from fluorescence readings within the central region of an amplification profile. However, a major challenge of implementing LRE quantification is the labor intensive nature of the analysis.
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机译：背景效率的线性回归（LRE）引入了实时qPCR的新范例，该范例通过消除对标准曲线的需求而实现了大规模绝对定量。通过将S形数学应用于基于SYBR Green I的测定法开发而成，目标量直接来自扩增曲线中心区域内的荧光读数。但是，实施LRE量化的主要挑战是分析的劳动密集型性质。
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