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Discrimination of Juvenile Yellowfin (Thunnus albacares) and Bigeye (T. obesus) Tunas using Mitochondrial DNA Control Region and Liver Morphology

机译:利用线粒体DNA控制区和肝形态学鉴别黄鳍金枪鱼和大眼金枪鱼金枪鱼

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摘要

Yellowfin tuna, Thunnus albacares (Bonnaterre, 1788) and bigeye tuna, Thunnus obesus (Lowe, 1839) are two of the most economically important tuna species in the world. However, identification of their juveniles, especially at sizes less than 40 cm, is very difficult, often leading to misidentification and miscalculation of their catch estimates. Here, we applied the mitochondrial DNA control region D-loop, a recently validated genetic marker used for identifying tuna species (Genus Thunnus), to discriminate juvenile tunas caught by purse seine and ringnet sets around fish aggregating devices (FADs) off the Southern Iloilo Peninsula in Central Philippines. We checked individual identifications using the Neighbor-Joining Method and compared results with morphometric analyses and the liver phenotype. We tested 48 specimens ranging from 13 to 31 cm fork length. Morpho-meristic analyses suggested that 12 specimens (25%) were bigeye tuna and 36 specimens (75%) were yellowfin tuna. In contrast, the genetic and liver analyses both showed that 5 specimens (10%) were bigeye tuna and 43 (90%) yellowfin tuna. This suggests that misidentification can occur even with highly stringent morpho-meristic characters and that the mtDNA control region and liver phenotype are excellent markers to discriminate juveniles of yellowfin and bigeye tunas.
机译:黄鳍金枪鱼,Thunnus albacares(Bonnaterre,1788)和大眼金枪鱼,Thunnus obesus(Lowe,1839)是世界上最重要的两种金枪鱼。但是,很难确定其幼鱼,特别是小于40厘米的幼鱼,常常导致误认和误算其捕捞估计数。在这里,我们应用了线粒体DNA控制区D-loop(一种最近被验证的用于鉴定金枪鱼物种(金枪鱼属)的遗传标记)来区分被伊洛伊洛南部鱼类聚集装置(FAD)周围的钱包围网和环网组捕获的少年金枪鱼。菲律宾中部半岛。我们使用Neighbor-Joining方法检查了个体识别,并将结果与​​形态分析和肝脏表型进行了比较。我们测试了48个样本,样本长度从13到31厘米不等。形态计量学分析表明,大眼金枪鱼有12个标本(占25%),黄鳍金枪鱼有36个标本(占75%)。相比之下,遗传和肝脏分析均显示5个标本(10%)是大眼金枪鱼,43个标本(90%)是黄鳍金枪鱼。这表明即使具有高度严格的形态异构特征,也可能发生错误识别,并且mtDNA控制区和肝表型是区分黄鳍金枪鱼和大眼金枪鱼幼体的极佳标记。

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