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首页> 美国卫生研究院文献>PLoS Clinical Trials >Testing the utility of DNA barcodes and a preliminary phylogenetic framework for Chinese freshwater mussels (Bivalvia: Unionidae) from the middle and lower Yangtze River
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Testing the utility of DNA barcodes and a preliminary phylogenetic framework for Chinese freshwater mussels (Bivalvia: Unionidae) from the middle and lower Yangtze River

机译：检验长江中下游中国淡水贻贝（双壳纲：Unionidae）的DNA条形码的实用性和初步的系统发育框架

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The middle and lower portions of the Yangtze River basin is the most species-rich region for freshwater mussels in Asia. The management and conservation of the taxa in this region has been greatly hampered by the lack of a well-developed phylogeny and species-level taxonomic framework. In this study, we tested the utility of two mitochondrial genes commonly used as DNA barcodes: the first subunit of the cytochrome oxidase c gene (COI) and the first subunit of the NADH dehydrogenase gene (ND1) for 34 putative species representing 15 genera, and also generated phylogenetic hypotheses for Chinese unionids based on the combined dataset of the two mitochondrial genes. The results showed that both loci performed well as barcodes for species identification, but the ND1 sequences provided better resolution when compared to COI. Based on the two-locus dataset, Bayesian Inference (BI) and Maximum Likelihood (ML) phylogenetic analyses indicated 3 of the 15 genera of Chinese freshwater mussels examined were polyphyletic. Additionally, the analyses placed the 15 genera into 3 subfamilies: Unioninae (Aculamprotula, Cuneopsis, Nodularia and Schistodesmus), Gonideninae (Lamprotula, Solenaia and Ptychorhychus) and Anodontinae (Cristaria, Arconaia, Acuticosta, Lanceolaria, Anemina and Sinoanodonta). Our results contradict previous taxonomic classification that placed the genera Arconaia, Acuticosta and Lanceolaria in the Unioninae. This study represents one of the first attempts to develop a molecular phylogenetic framework for the Chinese members of the Unionidae and will provide a basis for future research on the evolution, ecology, and conservation of Chinese freshwater mussels.
机译：长江流域的中下部是亚洲淡水贻贝物种最多的地区。由于缺乏完善的系统发育和物种水平的分类学框架，该地区的分类单元的管理和保护受到极大的阻碍。在这项研究中，我们测试了两个通常用作DNA条码的线粒体基因的实用性：细胞色素氧化酶c基因（COI）的第一个亚基和NADH脱氢酶基因（ND1）的第一个亚基，代表了15个属的34种假定种，并基于两个线粒体基因的综合数据集，为中华union提供了系统发育假说。结果表明，两个基因座都可作为条形码进行物种鉴定，但与COI相比，ND1序列可提供更好的分辨率。基于两基因座数据集，贝叶斯推断（BI）和最大似然（ML）系统发育分析表明，所研究的15个中国淡水贻贝属中的3个属于多系。此外，分析将15属分为3个亚科：Unioninae（Aculamprotula，Cuneopsis，结节菌和血吸虫），Gonideninae（Lamprotula，Solenaia和Ptychorhychus）和Anodontinae（Cristaria，Arconaia，Acuticosta，Lanceoldon，Anemina和Sinonota）。我们的结果与先前的分类学分类相矛盾，后者将Arconaia，Acuticosta和Lanceolaria属置于Unioninae。这项研究是为Union科中国成员开发分子系统发育框架的首次尝试之一，并将为今后有关中国淡水贻贝的进化，生态学和保护的研究提供基础。

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