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Effects of Glyphosate- Glufosinate- and Flazasulfuron-Based Herbicides on Soil Microorganisms in a Vineyard

机译:草甘膦草铵膦和基于草硫隆的除草剂对葡萄园土壤微生物的影响

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摘要

In a vineyard we examined the effects of broad-spectrum herbicides with three different active ingredients (glyphosate, glufosinate, flazasulfuron) on soil microorganisms. Mechanical weeding served as control treatment. Treatments were applied within grapevine rows and soil samples taken from there in 10–20 cm depth 77 days after application. Fungi were analyzed using classical sequencing technology and bacteria using next-generation sequencing. The number of colony-forming units (CFU) comprising bacteria, yeasts and molds was higher under flazasulfuron compared to all other treatments which had similar CFU levels. Abundance of the fungus Mucor was higher under flazasulfuron than glufosinate and mechanical weeding; Mucor was absent under glyphosate. Several other fungi taxa were exclusively found under a specific treatment. Up to 160 different bacteria species were found – some of them for the first time in vineyard soils. Total bacterial counts under herbicides were on average 260% higher than under mechanical weeding; however due to high variability this was not statistically significant. We suggest that herbicide-induced alterations of soil microorganisms could have knock-on effects on other parts of the grapevine system.
机译:在一个葡萄园中,我们研究了具有三种不同活性成分(草甘膦,草铵膦,黄酮磺隆)的广谱除草剂对土壤微生物的影响。机械除草作为对照处理。处理后在葡萄行中进行处理,并在施用后77天之内从10–20厘米深度处采集土壤样品。使用经典测序技术对真菌进行分析,使用下一代测序对细菌进行分析。与具有相似CFU水平的所有其他处理方法相比,在flazasulfuron中包含细菌,酵母和霉菌的菌落形成单位(CFU)的数量更高。在黄酮磺隆中,真菌Mucor的丰度高于草铵膦和机械除草。草甘膦下没有Mucor。仅在特定处理下发现了其他几种真菌类群。多达160种不同的细菌被发现-其中有些是第一次在葡萄园土壤中出现。除草剂下的细菌总数平均比机械除草下的细菌总数高260%;但是,由于高可变性,这在统计上并不显着。我们建议除草剂引起的土壤微生物改变可能会对葡萄树系统的其他部分产生连锁反应。

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