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首页> 美国卫生研究院文献>Springer Open Choice >Determination of cyanide in urine and saliva samples by ion chromatography with pulsed amperometric detection
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Determination of cyanide in urine and saliva samples by ion chromatography with pulsed amperometric detection

机译：脉冲安培检测-离子色谱法测定尿液和唾液中的氰化物

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AbstractCommonly known as a highly toxic chemical, cyanide is also an essential reagent for many industrial processes. It naturally occurs in plant seeds as cyanogenic glycosides. Another relatively common mode of cyanide exposure is inhalation of environmental tobacco smoke. This study concerns importance to determine cyanide ion in human biological samples. Urine and saliva samples were collected healthy volunteers exposed to tobacco smoke (active smokers) and environmental tobacco smoke (passive smokers). Chromatographic separation was achieved with an anion-exchange column and separated ions were detected by a pulsed amperometric detector. The method produced linear response in a specific concentration range of cyanide ion. The limit of detection was estimated at 0.1  and 0.5 µg/dm3 for urine and saliva samples, respectively. Cyanide ion concentrations in samples ranged from not detected (below LOD) to 12.88 µg/dm3. The comparison of results of biological samples analyses shows an increasing trend in cyanide concentration that may suggest that environmental tobacco smoke might have an impact on human health.
机译：摘要氰化物通常被称为剧毒化学品，也是许多工业过程中不可或缺的试剂。它天然存在于植物种子中，为氰苷。氰化物暴露的另一种相对常见的方式是吸入环境烟草烟雾。这项研究涉及确定人体生物样品中氰离子的重要性。收集暴露于烟草烟雾（主动吸烟者）和环境烟草烟雾（被动吸烟者）的健康志愿者的尿液和唾液样本。用阴离子交换柱进行色谱分离，并通过脉冲安培检测器检测分离的离子。该方法在特定的氰化物离子浓度范围内产生线性响应。尿液和唾液样品的检出限估计分别为0.1 µg / dm 3 。样品中的氰化物离子浓度范围从未检测到（低于检测限）到12.88 µg / dm 3 。对生物样品分析结果的比较表明，氰化物浓度呈上升趋势，这可能表明环境烟草烟雾可能对人类健康产生影响。

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