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Multi-platform metabolomics analyses of a broad collection of fragrant and non-fragrant rice varieties reveals the high complexity of grain quality characteristics

机译:对多种香型和非香型水稻品种的多平台代谢组学分析揭示了谷物品质特征的高度复杂性

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摘要

The quality of rice in terms not only of its nutritional value but also in terms of its aroma and flavour is becoming increasingly important in modern rice breeding where global targets are focused on both yield stability and grain quality. In the present paper we have exploited advanced, multi-platform metabolomics approaches to determine the biochemical differences in 31 rice varieties from a diverse range of genetic backgrounds and origin. All were grown under the specific local conditions for which they have been bred and all aspects of varietal identification and sample purity have been guaranteed by local experts from each country. Metabolomics analyses using 6 platforms have revealed the extent of biochemical differences (and similarities) between the chosen rice genotypes. Comparison of fragrant rice varieties showed a difference in the metabolic profiles of jasmine and basmati varieties. However with no consistent separation of the germplasm class. Storage of grains had a significant effect on the metabolome of both basmati and jasmine rice varieties but changes were different for the two rice types. This shows how metabolic changes may help prove a causal relationship with developing good quality in basmati rice or incurring quality loss in jasmine rice in aged grains. Such metabolomics approaches are leading to hypotheses on the potential links between grain quality attributes, biochemical composition and genotype in the context of breeding for improvement. With this knowledge we shall establish a stronger, evidence-based foundation upon which to build targeted strategies to support breeders in their quest for improved rice varieties.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-015-0925-1) contains supplementary material, which is available to authorized users.
机译:水稻的品质不仅在营养价值方面,而且在香气和风味方面都在现代水稻育种中变得越来越重要,在现代育种中,全球目标都集中在产量稳定性和谷物质量上。在本文中,我们已经利用先进的,多平台的代谢组学方法来确定来自不同遗传背景和起源的31个水稻品种的生化差异。它们均在适合其生长的特定当地条件下生长,并且各个国家的当地专家都保证了品种鉴定和样品纯度的各个方面。使用6个平台进行的代谢组学分析揭示了所选水稻基因型之间生化差异(和相似性)的程度。香米品种的比较显示茉莉和印度香米品种的代谢特征不同。但是,种质分类没有始终如一的分离。谷物的贮藏对印度香米和茉莉香米的代谢组都有重要影响,但两种水稻的变化却不同。这表明代谢变化如何有助于证明与印度香米的优质品质或茉莉香米的品质下降有关。这种代谢组学方法导致了关于在改良育种背景下谷物品质属性,生化成分和基因型之间潜在联系的假设。有了这些知识,我们将建立一个更强大的,循证的基础,在此基础上建立有针对性的策略来支持育种者寻求改良水稻品种。电子补充材料本文的在线版本(doi:10.1007 / s11306-015-0925-1 )包含补充材料,授权用户可以使用。

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