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首页> 美国卫生研究院文献>Springer Open Choice >Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism
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Genetic Diversity of the Edible Mushroom Pleurotus sp. by Amplified Fragment Length Polymorphism

机译：食用菌菇菇的遗传多样性。通过扩增片段长度多态性

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摘要

Pleurotus strains are the most important fungi used in the agricultural industry. The exact characterization and identification of Pleurotus species is fundamental for correct identification of the individuals and exploiting their full potential in food industry. The amplified fragment length polymorphism (AFLP) method was applied for genomic fingerprinting of 21 Pleurotus isolates of Asian and European origin. Using one PstI restriction endonuclease and four selective primers in an AFLP assay, 371 DNA fragments were generated, including 308 polymorphic bands. The AFLP profiles were found to be highly specific for each strain and they unambiguously distinguished 21 Pleurotus sp. fungi. The coefficient of Jaccard’s genome profile similarity between the analyzed strains ranged from 0.0 (Pleurotus sp. I vs. P. sajor-caju 237 and P. eryngii 238) to 0.750 (P. ostreatus 246 vs. P. ostreatus 248), and the average was 0.378. The AFLP-based dendrogram generated by the UPGMA method grouped all the Pleurotus fungi studied into two major clusters and one independent lineage located on the outskirt of the tree occupied by naturally growing Pleurotus species strain I. The results of the present study suggest the possible applicability of the AFLP-PstI method in effective identification and molecular characterization of Pleurotus sp. strains.
机译：平菇菌株是农业中最重要的真菌。杏鲍菇菌种的准确鉴定和鉴定对于正确鉴定个体和发挥其在食品工业中的全部潜力至关重要。应用扩增片段长度多态性（AFLP）方法对亚洲和欧洲来源的​​21个平菇分离株进行基因组指纹图谱分析。在AFLP分析中，使用一种PstI限制性核酸内切酶和四种选择性引物产生了371个DNA片段，包括308个多态性条带。发现AFLP图谱对每种菌株具有高度特异性，并且它们清楚地区分了21个侧耳属菌。菌类。所分析菌株之间的Jaccard基因组图谱相似性系数介于0.0（白灵菇I. vs. P. sajor-caju 237和P. eryngii 238）至0.750（P. ostreatus 246 vs. ostreatus 248）和平均值是0.378。通过UPGMA方法生成的基于AFLP的树状图将研究的所有侧耳真菌分为两个主要簇和一个独立谱系，它们位于自然生长的侧耳菌种I所占据的树的郊区。本研究结果表明了可能的适用性AFLP-PstI方法对白灵菇菌种的有效鉴定和分子表征株。

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