• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Springer Open Choice >Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli
【2h】

Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli

机译：侧流法中的碳纳米颗粒检测编码志贺毒素生产大肠杆菌致病因子的基因

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The use of carbon nanoparticles is shown for the detection and identification of different Shiga toxin-producing Escherichia coli virulence factors (vt1, vt2, eae and ehxA) and a 16S control (specific for E. coli) based on the use of lateral flow strips (nucleic acid lateral flow immunoassay, NALFIA). Prior to the detection with NALFIA, a rapid amplification method with tagged primers was applied. In the evaluation of the optimised NALFIA strips, no cross-reactivity was found for any of the antibodies used. The limit of detection was higher than for quantitative PCR (q-PCR), in most cases between 104 and 105 colony forming units/mL or 0.1–0.9 ng/μL DNA. NALFIA strips were applied to 48 isolates from cattle faeces, and results were compared to those achieved by q-PCR. E. coli virulence factors identified by NALFIA were in very good agreement with those observed in q-PCR, showing in most cases sensitivity and specificity values of 1.0 and an almost perfect agreement between both methods (kappa coefficient larger than 0.9). The results demonstrate that the screening method developed is reliable, cost-effective and user-friendly, and that the procedure is fast as the total time required is <1 h, which includes amplification.>FigureResults achieved with multi-analyte NALFIA for E. coli virulence factors. First strip: blank; second to sixth strip: each of the STEC factors; seventh strip: all factors
机译：显示了使用碳纳米颗粒检测和鉴定产生志贺毒素的不同大肠杆菌毒力因子（vt1，vt2，eae和ehxA）和基于侧向流动条的16S对照（针对大肠杆菌）。 （核酸侧流免疫测定，NALFIA）。在用NALFIA检测之前，应用了带有标记引物的快速扩增方法。在优化的NALFIA条的评估中，未发现任何所用抗体的交叉反应性。检测限高于定量PCR（q-PCR），在大多数情况下，菌落形成单位在10 4 和10 5 之间/ mL或0.1-0.9ng / μLDNA。将NALFIA条应用于牛粪的48个分离株，并将结果与​​q-PCR所得结果进行比较。通过NALFIA鉴定的大肠杆菌毒力因子与q-PCR中观察到的毒力因子非常吻合，表明在大多数情况下灵敏度和特异性值为1.0，并且两种方法之间的吻合度几乎完美（kappa系数大于0.9）。结果表明，开发的筛选方法可靠，经济高效且用户友好，并且由于所需的总时间为<1小时（包括扩增），因此该过程很快。<！-fig ft0-> <！ --fig @ position =“ anchor” mode =文章f4-> <！-fig mode =“ anchored” f5-> > Figure <！-fig / graphic | fig / alternatives / graphic mode =“锚定的“ m1-> <！-标题a7->使用多分析物NALFIA获得的大肠杆菌毒力因子结果。第一条：空白；第二至第六条：每个STEC因素；第七条：所有因素

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号