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Two Shiga toxin 2 subtypes in a single Shiga toxin-producing Escherichia coli analyzed by RT-qPCR, MALDI-TOF-TOF-MS and top-down proteomic analysis

机译：通过RT-QPCR，MALDI-TOF-TOF-MS和自上而下的蛋白质组学分析，两种Shiga毒素2种亚型分析的毒素毒素产生的大肠杆菌和自上而下的蛋白质组学分析

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Shiga toxin-producing Escherichia coli (STEC) are increasingly linked to outbreaks of foodborne illness worldwide. Shiga toxin (Stx) is an AB5 toxin with an A-subunit and five identical B-subunits. Amino acid sequence differences between Stx types and subtypes result in differences in toxicity. It is not uncommon for a STEC strain to carry multiple stx genes each with a different level of expression. We have measured the relative abundance of the B-subunits and mRNA transcripts of two Stx2 subtypes present in STEC strain E32511 using MALDI-TOF-TOF-MS and real time-quantitative polymerase chain reaction (RT-qPCR). Comparison of mRNA and toxin levels allow elucidation of whether toxin expression is under transcriptional control or translational control (or both).

机译：Shiga毒素生产的大肠杆菌（StEC）越来越多地与全世界食源食源病的爆发相关联。 Shiga毒素（STX）是AB5毒素，具有A-亚基和五个相同的B子单元。 STX类型和亚型之间的氨基酸序列差异导致毒性的差异。对于STEC菌株携带多个STX基因并且具有不同的表达水平并不罕见。我们使用MALDI-TOF-TOF-MS和实时定量聚合酶链反应（RT-QPCR）测量了STEC菌株E32511中存在的两个STX2亚型的B-亚基和mRNA转录物的相对丰度。 mRNA和毒素水平的比较允许阐明毒素表达是否处于转录控制或平移控制（或两者）。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2014年||共2页
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Clifton K. Fagerquist; William J. Zaragoza;
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1. Shiga Toxin 2 Subtypes of Enterohemorrhagic E. coli O157:H-E32511 Analyzed by RT-qPCR and Top-Down Proteomics Using MALDI-TOF-TOF-MS [J] . Fagerquist Clifton K., Zaragoza William J. Journal of the American Society for Mass Spectrometry . 2015,第5期

机译：RT-qPCR和自上而下蛋白质组学使用MALDI-TOF-TOF-MS分析的肠出血性大肠杆菌O157：H-E32511的志贺毒素2亚型
2. Top-Down Proteomic Identification of Shiga Toxin 2 Subtypes from Shiga Toxin-Producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization–Tandem Time of Flight Mass Spectrometry [J] . Clifton K. Fagerquist, William J. Zaragoza, Omar Sultan, Applied and Environmental Microbiology . 2014,第9期

机译：通过基质辅助激光解吸电离-串联飞行时间质谱从上至下的蛋白质组学鉴定产志贺毒素的大肠杆菌中的志贺毒素2亚型。
3. Top-Down Proteomic Identification of Shiga Toxin 2 Subtypes from Shiga Toxin-Producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization–Tandem Time of Flight Mass Spectrometry [J] . Clifton K. Fagerquist, William J. Zaragoza, Omar Sultan, Applied Microbiology . 2014,第9期

机译：通过基质辅助激光解吸电离-串联飞行时间质谱从上至下的蛋白质组学鉴定产志贺毒素的大肠杆菌中的志贺毒素2亚型。
4. Two Shiga toxin 2 subtypes in a single Shiga toxin-producing Escherichia coli analyzed by RT-qPCR, MALDI-TOF-TOF-MS and top-down proteomic analysis [C] . Clifton K. Fagerquist, William J. Zaragoza ASMS Conference on Mass Spectrometry and Allied Topics . 2014

机译：通过RT-QPCR，MALDI-TOF-TOF-MS和自上而下的蛋白质组学分析，两种Shiga毒素2种亚型分析的毒素毒素产生的大肠杆菌和自上而下的蛋白质组学分析
5. Diversity of Shiga toxin-converting phage and CRISPR loci in Shiga toxin-producing Escherichia coli: Evolutionary and pathogenicity implications. [D] . Yin, Shuang. 2014

机译：产生志贺毒素的大肠杆菌中转化志贺毒素的噬菌体和CRISPR基因座的多样性：进化和致病性意义。
6. Top-Down Proteomic Identification of Shiga Toxin 2 Subtypes from Shiga Toxin-Producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization–Tandem Time of Flight Mass Spectrometry [O] . Clifton K. Fagerquist, William J. Zaragoza, Omar Sultan, 2014

机译：通过基质辅助激光解吸电离-串联飞行时间质谱从上至下的蛋白质组学鉴定产志贺毒素的大肠杆菌中的志贺毒素2亚型。
7. Top-Down Proteomic Identification of Shiga Toxin 2 Subtypes from Shiga Toxin-Producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization-Tandem Time of Flight Mass Spectrometry [O] . C. K. Fagerquist, W. J. Zaragoza, O. Sultan, 2014

机译：通过基质辅助激光解吸电离 - 串联的飞行质谱法从Shiga毒素产生大肠杆菌的滋阴毒素2亚型的自上而下蛋白质组学鉴定

Two Shiga toxin 2 subtypes in a single Shiga toxin-producing Escherichia coli analyzed by RT-qPCR, MALDI-TOF-TOF-MS and top-down proteomic analysis

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