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首页> 美国卫生研究院文献>Springer Open Choice >Characterisation of the effect of a simulated hydrocarbon spill on diazotrophs in mangrove sediment mesocosm
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Characterisation of the effect of a simulated hydrocarbon spill on diazotrophs in mangrove sediment mesocosm

机译：表征模拟碳氢化合物溢流对红树林沉积物中膜重氮化物的影响

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An analysis of the effect of an oil spill on mangrove sediments was carried out by contamination of mesocosms derived from two different mangroves, one with a history of contamination and one pristine. The association between N2 fixers and hydrocarbon degradation was assessed using quantitative PCR (qPCR) for the genes rrs and nifH, nifH clone library sequencing and total petroleum hydrocarbon (TPH) quantification using gas chromatography. TPH showed that the microbial communities of both mangroves were able to degrade the hydrocarbons added; however, whereas the majority of oil added to the mesocosm derived from the polluted mangrove was degraded in the 75 days of the experiment, there was only partially degradation in the mesocosm derived from the pristine mangrove. qPCR showed that the addition of oil led to an increase in rrs gene copy numbers in both mesocosms, having almost no effect on the nifH copy numbers in the pristine mangrove. Sequencing of nifH clones indicated that the changes promoted by the oil in the polluted mangrove were greater than those observed in the pristine mesocosm. The main effect observed in the polluted mesocosm was the selection of a single phylotype which is probably adapted to the presence of petroleum. These results, together with previous reports, give hints about the relationship between N2 fixation and hydrocarbon degradation in natural ecosystems.
机译：对溢油对红树林沉积物的影响进行了分析，方法是污染源自两种不同红树林的中观，其中一种具有污染历史，一种原始。使用定量PCR（qPCR）评估rrs和nifH基因，nifH克隆文库测序以及使用气相色谱法测定总石油烃（TPH）数量，评估N2固定剂与烃降解之间的关联。 TPH显示，两种红树林的微生物群落都能够降解添加的碳氢化合物。然而，尽管在实验的75天中，添加到源自受污染红树林的中膜的大部分油都已降解，但源自原始红树林的中膜却仅部分降解。 qPCR显示，添加油会导致两个中膜的rrs基因拷贝数增加，而对原始红树林的nifH拷贝数几乎没有影响。 nifH克隆的测序表明，油在受污染的红树林中促进的变化大于在原始中观世界中观察到的变化。在受污染的中观世界中观察到的主要作用是选择了一个可能适合石油存在的单一系统型。这些结果以及以前的报告提供了关于自然生态系统中N2固定与碳氢化合物降解之间关系的暗示。

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