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首页> 美国卫生研究院文献>Taylor Francis Open Select >Intraspecific protoplast fusion of Brettanomyces anomalus for improved production of an extracellular β-glucosidase
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Intraspecific protoplast fusion of Brettanomyces anomalus for improved production of an extracellular β-glucosidase

机译：异常酒香酵母的种内原生质体融合提高细胞外β-葡萄糖苷酶的产量

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摘要

Improvement of production of an extracellular β-glucosidase with high activity by Brettanomyces anomalus PSY-001 was performed by using recursive protoplast fusion in a genome-shuffling format. The initial population was generated by ultraviolet irradiation, ultrasonic mutagenesis and, then, subjected to recursive protoplast fusion. Mutant strains exhibiting significantly higher β-glucosidase activities in liquid media were isolated. The best mutant strain showed increased cell growth in a flask culture, as well as increased β-glucosidase production. A recombinant strain, F3-25, was obtained after three rounds of genome shuffling and its production of β-glucosidase activity reached 4790 U L−1, which was a nearly eightfold increase compared to the original strain B. anomalus PSY-001. The subculture experiments indicated that F3-25 was genetically stable.
机译：通过使用基因组改组形式的递归原生质体融合来实现由异常酵母（Brettanomyces anomalus PSY-001）提高的具有高活性的细胞外β-葡萄糖苷酶的生产。初始种群是通过紫外线照射，超声诱变产生的，然后进行递归原生质体融合。分离出在液体培养基中表现出明显更高的β-葡萄糖苷酶活性的突变菌株。最好的突变菌株在烧瓶培养物中显示出增加的细胞生长，以及增加的β-葡萄糖苷酶产量。经过三轮基因组改组后获得了重组菌株F3-25，其β-葡萄糖苷酶活性产生达到4790 UL -1 ，与原始菌株B相比增加了近八倍。异常PSY-001。继代培养实验表明F3-25是遗传稳定的。

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