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Overexpression of O‐polysaccharide chain length regulators in Gram‐negative bacteria using the Wzx‐/Wzy‐dependent pathway enhances production of defined modal length O‐polysaccharide polymers for use as haptens in glycoconjugate vaccines

机译：使用Wzx // Wzy依赖性途径在革兰氏阴性细菌中过表达O-多糖链长调节剂可提高确定的模态长度O-多糖聚合物的产量这些聚合物可用作糖缀合物疫苗中的半抗原

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AimsO‐polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines. Variability in the OPS chain length is one obstacle towards further development of these vaccines. Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield. The overall goal of this study was to demonstrate the utility of engineering Gram‐negative bacteria to produce homogenous O‐polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O‐polysaccharide chain length regulators of the Wzx‐/Wzy‐dependent pathway.

机译：AimsO-多糖（OPS）分子是几种细菌病原体的保护性抗原，作为糖缀合物疫苗的成分具有广泛的用途。 OPS链长度的可变性是进一步开发这些疫苗的障碍之一。在纯化次优或混合长度的OPS分子期间引入上浆步骤会增加成本和复杂性，同时降低最终产量。这项研究的总体目标是证明工程革兰氏阴性细菌通过过表达Wzx // Wzy依赖的O-多糖链长度调节剂来生产均一的O-多糖种群的实用性，可将其用作碳水化合物疫苗的基础途径。

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期刊名称 Wiley-Blackwell Online Open
作者
N. Hegerle; J. Bose; G. Ramachandran; J.E. Galen; M.M. Levine; R. Simon; S.M. Tennant;
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作者单位

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年(卷),期 -1(125),2
年度 -1
页码 575–585
总页数 11
原文格式 PDF
正文语种
中图分类
关键词
glycoconjugate haptens LPS (Lipopolysaccharide) O‐polysaccharide pseudomonas salmonella vaccines;

机译：糖缀合物;半抗原;LPS（脂多糖）;O-多糖;假单胞菌;沙门氏菌;疫苗;

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专利

1. Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines [J] . Hegerle N., Bose J., Ramachandran G., Journal of applied microbiology . 2018,第2期

机译：使用WZX-/ WZY依赖性途径的革兰阴性细菌中O-多糖链长度调节剂的过度表达增强了定义的模态长度O-多糖聚合物的产生，以用作糖凝胶疫苗中的耐高温
2. Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway [J] . International Journal of Biological Macromolecules: Structure, Function and Interactions . 2020,第期

机译：Escherichia Albertii EA046（O9）Harbors两种多糖基因簇，用于通过WZM / WZT依赖性途径由大肠杆菌o8共享的o-antigen合成O-抗原的簇
3. Synthesis of bacterial polysaccharides via the Wzx/Wzy-dependent pathway [J] . Islam Salim T., Lam Joseph S. Canadian journal of microbiology . 2014,第11期

机译：通过Wzx / Wzy依赖途径合成细菌多糖
4. Note: The Modality of Enterobacterial Common Antigen Polysaccharide Chain Lengths Is Regulated by o349 of the wec Gene Cluster of Escherichia coli K-12 [O] . Kathleen Barr, John Klena, Paul D. Rick 1999

机译：注意：肠细菌常见抗原多糖链长度的模式由大肠杆菌K-12的wec基因簇的o349调节。
5. Overexpression of O‐polysaccharide chain length regulators in Gram‐negative bacteria using the Wzx‐/Wzy‐dependent pathway enhances production of defined modal length O‐polysaccharide polymers for use as haptens in glycoconjugate vaccines [O] . N. Hegerle, J. Bose, G. Ramachandran, 2018

机译：使用WZX / WZY依赖性途径的革兰阴性细菌中O-多糖链长度调节剂的过度表达增强了用于糖凝胶疫苗中定义的模态长度O-多糖聚合物的产生

Overexpression of O‐polysaccharide chain length regulators in Gram‐negative bacteria using the Wzx‐/Wzy‐dependent pathway enhances production of defined modal length O‐polysaccharide polymers for use as haptens in glycoconjugate vaccines

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