Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines

Hegerle N.; Bose J.; Ramachandran G.; Galen J. E.; Levine M. M.; Simon R.; Tennant S. M.

首页> 外文期刊>Journal of applied microbiology >Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines

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Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines

机译：使用WZX-/ WZY依赖性途径的革兰阴性细菌中O-多糖链长度调节剂的过度表达增强了定义的模态长度O-多糖聚合物的产生，以用作糖凝胶疫苗中的耐高温

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AimsO-polysaccharide (OPS) molecules are protective antigens for several bacterial pathogens, and have broad utility as components of glycoconjugate vaccines. Variability in the OPS chain length is one obstacle towards further development of these vaccines. Introduction of sizing steps during purification of OPS molecules of suboptimal or of mixed lengths introduces additional costs and complexity while decreasing the final yield. The overall goal of this study was to demonstrate the utility of engineering Gram-negative bacteria to produce homogenous O-polysaccharide populations that can be used as the basis of carbohydrate vaccines by overexpressing O-polysaccharide chain length regulators of the Wzx-/Wzy-dependent pathway.

机译：AIMSO-多糖（OPS）分子是几种细菌病原体的保护性抗原，并作为甘油杂化植物疫苗的组分具有广泛的效用。 OPS链长度的变异性是朝向这些疫苗进一步发展的一个障碍。在次优或混合长度的OPS分子纯化期间引入施胶步骤引入了额外的成本和复杂性，同时降低了最终产量。本研究的整体目标是证明工程革兰氏阴性细菌的效用，以产生均匀的O-多糖群，可以通过过表达WZX-/ WZY依赖性的O-多糖链长度调节剂作为碳水化合物疫苗的基础途径。

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来源
《Journal of applied microbiology》 |2018年第2期|共11页
作者
Hegerle N.; Bose J.; Ramachandran G.; Galen J. E.; Levine M. M.; Simon R.; Tennant S. M.;
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作者单位

Univ Maryland Sch Med Ctr Vaccine Dev 685 W Baltimore St HSF Room 480 Baltimore MD 21201 USA;

Univ Maryland Sch Med Ctr Vaccine Dev 685 W Baltimore St HSF Room 480 Baltimore MD 21201 USA;

Univ Maryland Sch Med Ctr Vaccine Dev 685 W Baltimore St HSF Room 480 Baltimore MD 21201 USA;

Univ Maryland Sch Med Ctr Vaccine Dev 685 W Baltimore St HSF Room 480 Baltimore MD 21201 USA;

Univ Maryland Sch Med Ctr Vaccine Dev 685 W Baltimore St HSF Room 480 Baltimore MD 21201 USA;

Univ Maryland Sch Med Ctr Vaccine Dev 685 W Baltimore St HSF Room 480 Baltimore MD 21201 USA;

Univ Maryland Sch Med Ctr Vaccine Dev 685 W Baltimore St HSF Room 480 Baltimore MD 21201 USA;

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原文格式 PDF
正文语种 eng
中图分类应用微生物学;
关键词
glycoconjugate; haptens; LPS (Lipopolysaccharide); O-polysaccharide; pseudomonas; salmonella; vaccines;

机译：glycoconjugate;哈维特;LPS（脂多糖）;O-多糖;假单胞菌;沙门氏菌;疫苗;

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1. Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines [J] . Hegerle N., Bose J., Ramachandran G., Journal of applied microbiology . 2018,第2期

机译：使用WZX-/ WZY依赖性途径的革兰阴性细菌中O-多糖链长度调节剂的过度表达增强了定义的模态长度O-多糖聚合物的产生，以用作糖凝胶疫苗中的耐高温
2. MODULATION OF THE SURFACE ARCHITECTURE OF GRAM-NEGATIVE BACTERIA BY THE ACTION OF SURFACE POLYMER - LIPID A-CORE LIGASE AND BY DETERMINANTS OF POLYMER CHAIN LENGTH [Review] [J] . Whitfield C., Koplin R., Amor PA. Molecular Microbiology . 1997,第4期

机译：表面聚合物-脂质A-核心连接酶的作用以及聚合物链长的决定因素对革兰氏阴性细菌表面结构的调节[综述]
3. Overexpression of O‐polysaccharide chain length regulators in Gram‐negative bacteria using the Wzx‐/Wzy‐dependent pathway enhances production of defined modal length O‐polysaccharide polymers for use as haptens in glycoconjugate vaccines [O] . N. Hegerle, J. Bose, G. Ramachandran, -1

机译：使用Wzx // Wzy依赖性途径在革兰氏阴性细菌中过表达O-多糖链长调节剂可提高确定的模态长度O-多糖聚合物的产量这些聚合物可用作糖缀合物疫苗中的半抗原
4. Overexpression of O‐polysaccharide chain length regulators in Gram‐negative bacteria using the Wzx‐/Wzy‐dependent pathway enhances production of defined modal length O‐polysaccharide polymers for use as haptens in glycoconjugate vaccines [O] . N. Hegerle, J. Bose, G. Ramachandran, 2018

机译：使用WZX / WZY依赖性途径的革兰阴性细菌中O-多糖链长度调节剂的过度表达增强了用于糖凝胶疫苗中定义的模态长度O-多糖聚合物的产生

Overexpression of O-polysaccharide chain length regulators in Gram-negative bacteria using the Wzx-/Wzy-dependent pathway enhances production of defined modal length O-polysaccharide polymers for use as haptens in glycoconjugate vaccines

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