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首页> 美国卫生研究院文献>Wiley-Blackwell Online Open >Raman spectroscopy for accurately characterizing biomolecular changes in androgen‐independent prostate cancer cells
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Raman spectroscopy for accurately characterizing biomolecular changes in androgen‐independent prostate cancer cells

机译：拉曼光谱法可准确表征雄激素非依赖性前列腺癌细胞中的生物分子变化

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Metastatic prostate cancer resistant to hormonal manipulation is considered the advanced stage of the disease and leads to most cancer‐related mortality. With new research focusing on modulating cancer growth, it is essential to understand the biochemical changes in cells that can then be exploited for drug discovery and for improving responsiveness to treatment. Raman spectroscopy has a high chemical specificity and can be used to detect and quantify molecular changes at the cellular level. Collection of large data sets generated from biological samples can be employed to form discriminatory algorithms for detection of subtle and early changes in cancer cells. The present study describes Raman finger printing of normal and metastatic hormone‐resistant prostate cancer cells including analyses with principal component analysis and linear discrimination. Amino acid‐specific signals were identified, especially loss of arginine band. Androgen‐resistant prostate cancer cells presented a higher content of phenylalanine, tyrosine, DNA and Amide III in comparison to PNT2 cells, which possessed greater amounts of L‐arginine and had a B conformation of DNA. The analysis utilized in this study could reliably differentiate the 2 cell lines (sensitivity 95%; specificity 88%).
机译：抵抗激素操纵的转移性前列腺癌被认为是该病的晚期阶段，可导致大多数与癌症相关的死亡。随着专注于调节癌症生长的新研究的深入，必须了解细胞中的生化变化，然后可以将其用于药物发现和改善对治疗的反应性。拉曼光谱法具有很高的化学特异性，可用于检测和量化细胞水平的分子变化。从生物样品中产生的大数据集的收集可用于形成区分性算法，以检测癌细胞的细微变化和早期变化。本研究描述了正常和转移性激素抵抗性前列腺癌细胞的拉曼指纹，包括具有主成分分析和线性判别的分析。鉴定出氨基酸特异性信号，尤其是精氨酸带的丢失。与PNT2细胞相比，抗雄激素的前列腺癌细胞的苯丙氨酸，酪氨酸，DNA和酰胺III含量更高，后者的L-精氨酸含量更高，DNA具有B构象。这项研究中使用的分析可以可靠地区分2种细胞系（敏感性95％；特异性88％）。

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