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首页> 美国政府科技报告 >Cell Cycle Regulation in Prostate Cancer and its Role in the Transition of Androgen-Dependent Prostate Cancer to Androgen-Independency.
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Cell Cycle Regulation in Prostate Cancer and its Role in the Transition of Androgen-Dependent Prostate Cancer to Androgen-Independency.

机译：前列腺癌细胞周期调控及其在雄激素依赖性前列腺癌向雄激素依赖性转变中的作用。

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Cell proliferation in the prostate is dependent upon androgen and is associated with specific cell cycle alterations. To examine the relation between cell cycle regulation and androgen-induced proliferation in prostate cancer the transferable human prostate tumor MDA Pca2b was employed. This cell line is androgen-independent but has shown to proliferate in response to androgen. Pca2b tumors were grown in nude mice and when tumors had reached a size of 0.5 cm tumor-bearing animals were castrated. At 3 weeks after castration mice were injected daily with testosterone propionate to replace androgen. Tumor tissue was collected from intact mice, from castrated mice at several time points after castration and from castrated, androgen-treated mice at several time points after start of androgen-replacement. Protein isolated from the tumor samples was subjected to western blot analysis. Cyclins D1, A, and E, cdk 2 and 4, p21 and p27 were present at detectable levels in Pca2b. No significant changes in the levels of these cell cycle proteins were detected upon castration or androgen replacement. It was concluded that the androgen-induced proliferative response of MDA Pca2b is not mediated via the cell cycle pathway.

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