目的 研究淫羊藿苷对BALB/c-nu裸小鼠雄激素依赖性前列腺癌雄激素受体信号转导通路(P13K/Akt)的影响.方法将40只雄性BALB/c-nu裸小鼠随机均分为对照组、模型组、抑制剂组和实验组(n=10),除对照组外均采用前列腺内注射人前列腺癌细胞株(LNCaP)悬液(2×107个/mL)的方法建立LNCaP模型,2周后,4组均采用尾静脉注射相应药物的方法治疗28 d.采用Western blotting法检测前列腺癌变组织雄激素受体剪接变异体7(AR-V7)、磷酸化蛋白激酶(p-Akt)和磷酸化雄激素受体(p-AR)蛋白表达;FITC-CY3法检测钙黏蛋白E(E-cadherin)和降钙素(Calcitonin)阳性率.结果与模型组比较,实验组p-Akt及p-AR蛋白均低表达(P<0.05),而Calctionin及AR-V7的阳性率均明显降低(P<0.05),E-cadherin的阳性率明显升高(P<0.05).结论淫羊藿苷通过调控P13K/Akt信号转导通路相关因子表达而抑制LNCaP侵袭和转移.%Objective To study the effects of effects of icariin on signaling pathway of androgen receptor (P13K/Akt) of androgen-dependent BALB/c-nu nude mice with prostate cancer.Methods Forty male BALB/c-nu nude mice were randomly divided into control group, model group, inhibitor group and experimental group (n=10). Expect control group, suspension (2×107 cells/mL) of prostate cancer cells (LNCaP) were injected into the other groups, thus to establish the LNCaP model. Two weeks later, the four groups were all treated with injections of the corresponding drugs for 28 days through tail vein. Western blotting was used to detect the expressions of androgen receptor splicing variant 7 (AR-V7), phosphorylated protein kinase (p-Akt) and phosphorylated androgen receptor (p-AR) protein in prostate cancer; FITC-CY3 method was used to test the positive rates of E-cadherin and calcitonin.Results Compared with the model group, the expressions of p-Akt and p-AR protein in the experimental group were lower (P<0.05), and the positive rates of Calctionin and AR-V7 were significantly lower (P<0.05), but the positive rate of E-cadherin was significantly higher. (P<0.05).Conclusion Icariin could inhibit the invasion and metastasis of LNCaP by regulating the expressions of related factors in P13K/Akt signaling pathway.
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