• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Wiley-Blackwell Online Open >Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana and functional and molecular comparisons between AS2 and other family members
【2h】

Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana and functional and molecular comparisons between AS2 and other family members

机译：拟南芥不对称叶2 /侧向器官边界（AS2 / LOB）家族中基因的表征以及AS2与其他家族成员之间的功能和分子比较

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The ASYMMETRIC LEAVES2 (AS2) gene is required for the generation of the flat and symmetrical shape of the leaf lamina in Arabidopsis. AS2 encodes a plant-specific protein with an AS2/LATERAL ORGAN BOUNDARIES (AS2/LOB) domain that includes a cysteine repeat, a conserved single glycine residue and a leucine-zipper-like sequence in its amino-terminal half. The Arabidopsis genome contains 42 genes, including AS2, that encode proteins with an AS2/LOB domain in their amino-terminal halves, and these genes constitute the AS2/LOB gene family. In the present study, we cloned and characterized cDNAs that covered the putative coding regions of all members of this family, and investigated patterns of transcription systematically in Arabidopsis plants. Comparisons among amino acid sequences that had been deduced from the cloned cDNAs revealed eight groups of genes, with two or three members each, and high degrees of identity among entire amino acid sequences, suggesting that some members of the AS2/LOB family might have redundant function(s). Moreover, no member of the family exhibited significant similarity, in terms of the deduced amino acid sequence of the carboxy-terminal half, to AS2. Results of domain swapping between AS2 and other members of the family showed that the AS2/LOB domain of AS2 cannot be functionally replaced by those of other members of the family, and that only a few dissimilarities among respective amino acid residues of the AS2/LOB domain of AS2 and those of other members are important for the specific functions of AS2.
机译：ASYMMETRIC LEAVES2（AS2）基因是拟南芥中叶片层状扁平而对称形状的生成所必需的。 AS2编码具有AS2 / LATERAL ORGAN BOUNDARIES（AS2 / LOB）域的植物特异性蛋白质，该域在其氨基末端一半包含半胱氨酸重复序列，保守的单个甘氨酸残基和亮氨酸拉链样序列。拟南芥基因组包含42个基因（包括AS2），这些基因编码在氨基末端一半带有AS2 / LOB结构域的蛋白质，这些基因构成AS2 / LOB基因家族。在本研究中，我们克隆并鉴定了覆盖该家族所有成员推定编码区的cDNA，并系统地研究了拟南芥植物中的转录模式。从克隆的cDNA推导的氨基酸序列之间的比较揭示了八组基因，每组有两个或三个成员，并且在整个氨基酸序列之间具有高度同一性，这表明AS2 / LOB家族的某些成员可能具有冗余性功能）。此外，就推导的羧基末端一半的氨基酸序列而言，该家族的成员均未表现出与AS2的显着相似性。 AS2与该家族其他成员之间的域交换结果显示，AS2的AS2 / LOB结构域不能在功能上被该家族其他成员取代，并且AS2 / LOB各自氨基酸残基之间只有很少的差异AS2和其他成员的域对于AS2的特定功能很重要。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号