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首页> 外文期刊>Acta Physiologiae Plantarum >Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture
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Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture

机译：紫花苜蓿（Lehm）的微繁殖。 DC。愈伤组织和细胞悬浮培养法制备链烷烃

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摘要

Alkannin, a red-purple dye and bioactive compound found in the roots of Arnebia hispidissima has antibiotic and anti-inflammatory properties and is also used in cosmetic and textile industries at a large-scale. In the present communication, we demonstrate the establishment of callus and cell suspension culture of A. hispidissima with the aim of optimizing the production of alkannin. Highest alkannin content was recorded in cell suspension and callus culture established on M-9 medium. Production of alkannin was influenced by the different culture medium. Evaluation of alkannin content of roots of field-grown plants and in vitro grown cell, tissue and organ showed that alkannin production was higher in all in vitro grown culture systems (cell suspension, callus and roots) than the roots of field-grown plants. The present investigation may be applicable in designing systems for the large-scale cultivation of A. hispidissima cell suspensions for the production of alkannin.
机译：紫花苜蓿，一种在紫花苜蓿的根中发现的红紫色染料和生物活性化合物，具有抗生素和抗炎特性，还广泛用于化妆品和纺织工业。在本次交流中，我们证明了拟南芥的愈伤组织和细胞悬浮培养的建立，旨在优化链烷烃的生产。在细胞悬浮液和在M-9培养基上建立的愈伤组织培养物中记录到最高的链烷烃含量。链烷素的生产受不同培养基的影响。对田间生长植物的根以及体外生长的细胞，组织和器官的链烷烃含量的评估表明，在所有体外生长的培养系统（细胞悬浮液，愈伤组织和根）中，链烷烃的产量均高于田间生长的植物的根。本研究可能适用于大规模生产链霉菌生产链霉菌悬浮细胞的设计系统。

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