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首页> 外文期刊>Acta Physiologiae Plantarum >Isolation and characterisation of a cDNA encoding a novel cytosolic ascorbate peroxidase from potato plants (Solanum tuberosum L.)
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Isolation and characterisation of a cDNA encoding a novel cytosolic ascorbate peroxidase from potato plants (Solanum tuberosum L.)

机译：马铃薯植物（Solanum tuberosum L.）编码新型胞质抗坏血酸过氧化物酶的cDNA的分离与鉴定

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摘要

Ascorbate peroxidase (APX) is one of the key enzymes of the plant antioxidant system playing, along with catalase, a central role in hydrogen peroxide scavenging. An approach to further increase the knowledge about cytosolic APX gene organization can be achieved by isolating and characterisating new cDNAs, thus providing new insights about the physiological roles and regulation of these enzymes. A partial cDNA clone (corresponding to the 3’ untranslated region), cytosolic ascorbate peroxidase-related, was isolated from potato sprouts by RT-PCR. Database analysis retrieved several expressed sequence tags (ESTs) coding potato cytosolic ascorbate peroxidase, that were used to infer the complete cDNA sequence. The deduced amino acid sequence revealed high homologies with other plant cytosolic ascorbate peroxidases, confirming the reliability of the virtual cDNA. Northern blot analysis revealed the existence of a single band related to the isolated cDNA and the southern blotting results allowed the elaboration of a possible gene organization.
机译：抗坏血酸过氧化物酶（APX）是植物抗氧化系统的关键酶之一，过氧化氢酶与过氧化氢酶一起发挥着重要作用。可以通过分离和表征新的cDNA来获得进一步增加有关胞质APX基因组织知识的方法，从而提供有关这些酶的生理作用和调节的新见解。通过RT-PCR从马铃薯芽中分离出部分cDNA克隆（对应于3'非翻译区），胞质抗坏血酸过氧化物酶相关。数据库分析检索了编码马铃薯胞质抗坏血酸过氧化物酶的几种表达序列标签（EST），这些序列标签可用来推断完整的cDNA序列。推导的氨基酸序列显示出与其他植物胞质抗坏血酸过氧化物酶的高度同源性，证实了虚拟cDNA的可靠性。 Northern印迹分析揭示了与分离的cDNA相关的单个条带的存在，并且Southern印迹结果允许阐述可能的基因组织。

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  • 来源
    《Acta Physiologiae Plantarum》 |2006年第1期|41-47|共7页
  • 作者

    Diana Soares da Costa; Cláudia Sofia Pereira; Jorge Teixeira; Susana Pereira;

  • 作者单位

    Plant Functional Biology Department Institute for Cell and Molecular Biology University of Porto;

    Plant Functional Biology Department Institute for Cell and Molecular Biology University of Porto;

    Plant Functional Biology Department Institute for Cell and Molecular Biology University of PortoBotany Department School of Sciences University of Porto Rua do Campo Alegre;

    Plant Functional Biology Department Institute for Cell and Molecular Biology University of PortoBotany Department School of Sciences University of Porto Rua do Campo Alegre;

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  • 正文语种 eng
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    ascorbate peroxidase; cDNA isolation; northern blotting; potato; southern blotting;

    机译：抗坏血酸过氧化物酶;cDNA分离;北方印迹;马铃薯;南方印迹;

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