• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Organ-specific distribution and subcellular localisation of ascorbate peroxidase isoenzymes in potato (Solanum tuberosum L.) plants
【24h】

Organ-specific distribution and subcellular localisation of ascorbate peroxidase isoenzymes in potato (Solanum tuberosum L.) plants

机译：马铃薯（Solanum tuberosum L.）植物中抗坏血酸过氧化物酶同工酶的器官特异性分布和亚细胞定位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ascorbate peroxidase (EC 1.11.1.11), a heme-containing homodimeric protein, is a hydrogen peroxide-scavenging enzyme, playing an important role in plants in order to protect them from oxidative stress, thus adverting cellular damage. Several ascorbate peroxidase isoenzymes have been reported but the understanding of their physiological role still depends on a better knowledge of their precise localisation within plant organs. Immunocytochemistry techniques were performed in order to elucidate the peroxisomal and cytosolic ascorbate peroxidase distribution within tissues of leaves and sprouts of potato plants. The peroxisomal isoenzyme was found to have a broad distribution in sprouts, but a differential one in leaves, being restricted to the spongy parenchyma. This differential expression may be associated to the mesophyll asymmetry and the diverse physiological processes that occur in it. The cytosolic isoenzyme was not detected in leaves under the used conditions, probably because it is present in low amounts in these tissues. The results obtained in sprouts were at least curious: cytosolic ascorbate was found to be adjacent to the amyloplasts. Given these results, it is possible to state that apart from their similarity, these two isoenzymes reside in different organelles and seem to take part in different physiological processes as suggested by their organ- and tissue-specific distribution.
机译：抗坏血酸过氧化物酶（EC 1.11.1.11）是一种含血红素的同型二聚体蛋白，是一种过氧化氢清除酶，在植物中起着重要作用，以保护植物免受氧化胁迫，从而破坏细胞。已经报道了几种抗坏血酸过氧化物酶同工酶，但是对它们的生理作用的理解仍然取决于对它们在植物器官中的精确定位的更好的了解。进行了免疫细胞化学技术，以阐明马铃薯植物叶片和新芽组织中的过氧化物酶体和胞质抗坏血酸过氧化物酶分布。发现过氧化物酶体同工酶在新芽中分布较广，但在叶片中却有差异，只限于海绵状薄壁组织。这种差异表达可能与叶肉不对称和其中发生的多种生理过程有关。在使用条件下，未在叶片中检测到胞质同工酶，可能是因为它们在这些组织中的含量很低。在豆芽中获得的结果至少是令人好奇的：发现胞质抗坏血酸与淀粉质体相邻。鉴于这些结果，有可能指出，除了它们的相似性以外，这两种同工酶还存在于不同的细胞器中，并且由于它们的器官和组织特异性分布而似乎参与了不同的生理过程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号