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首页> 外文期刊>Acta Physiologiae Plantarum >Expressed sequence tags and their application for plant research
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Expressed sequence tags and their application for plant research

机译:表达序列标签及其在植物研究中的应用

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摘要

Expressed Sequence Tags (ESTs) are short, usually unedited sequences obtained by single-pass sequencing of cDNA clones from any cDNA library. Analyzing and comparing ESTs can provide information on gene expression, function and evolution. Large-scale EST sequencing has become an attractive alternative to plant genome sequencing. Currently, plant EST collections comprise over 3.8 million sequences from about 200 species. They have proved to be a valuable tool for gene discovery and plant metabolism analysis. Several plant-specific EST databases have been created which provide access to sequence data and bioinformatics-based tools for data mining. Searching EST collections allows pre-selection of genes for preparing cDNA arrays, targeted to bring maximum information on specialized processes, like stress response, symbiotic nitrogen fixation etc. Also, ESt-based molecular markers such as SNP, SSR, and indels are fast developing tools for breeders and researchers.
机译:表达序列标签(EST)是简短的,通常未经编辑的序列,是通过对来自任何cDNA文库的cDNA克隆进行单遍测序而获得的。分析和比较EST可以提供有关基因表达,功能和进化的信息。大规模EST测序已成为植物基因组测序的一种有吸引力的替代方法。目前,植物EST集合包含来自约200个物种的380万个序列。它们已被证明是用于基因发现和植物代谢分析的有价值的工具。已经创建了几个特定于工厂的EST数据库,这些数据库提供对序列数据和基于生物信息学的工具进行数据挖掘的访问权限。通过搜索EST集合,可以预先选择用于制备cDNA阵列的基因,目的是在特定过程中提供最大信息,例如应激反应,共生固氮等。而且,基于ESt的分子标记(例如SNP,SSR和indels)正在快速发展育种人员和研究人员的工具。

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