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A simple and efficient method for cryopreservation of embryogenic triticale calli

机译:一种简单有效的冷冻胚性黑小麦愈伤组织的方法

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摘要

Conventional breeding methods are now supplemented by modern in vitro techniques. However, long term maintenance of cultures has many disadvantages. It incurs risk of loss through microbial contamination, somaclonal variation or human error, but above all the regeneration capacity can decrease gradually during extended maintenance. Cryopreservation as a method of long term storage of biological material without genetic alteration was adapted for embryogenic triticale calli preservation. Callus of both winter and spring genotypes were successfully cryopreserved. The best viability rates (80–85%) were achieved with 6 weeks old winter genotypes treated with cryoprotective solution containing DMSO. This simple and efficient method of cryopreservation requires no special devices for controlled freezing and can be easily adapted for other cereals.
机译:现在,现代的体外技术补充了常规的育种方法。但是,长期维持文化有许多弊端。它会因微生物污染,体​​细胞克隆变异或人为失误而造成损失的风险,但最重要的是,在长期维护期间,再生能力会逐渐降低。冷冻保存作为一种无需遗传改变即可长期保存生物材料的方法,适用于胚胎发生黑小麦愈伤组织的保存。冻存了冬季和春季基因型的愈伤组织。用含DMSO的防冻溶液处理的6周龄的冬季基因型可达到最佳生存率(80-85%)。这种简单而有效的冷冻保存方法不需要用于控制冷冻的特殊装置,并且可以轻松地用于其他谷物。

著录项

  • 来源
    《Acta Physiologiae Plantarum》 |2005年第2期|237-243|共7页
  • 作者

    S. Sowa; S. Oleszczuk; J. Zimny;

  • 作者单位

    Plant breeding and Acclimatization Institute Radzików Poland;

    Plant breeding and Acclimatization Institute Radzików Poland;

    Plant breeding and Acclimatization Institute Radzików Poland;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cryopreservation; triticale; callus;

    机译:冷冻保存;黑小麦;愈伤组织;

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