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首页> 外文期刊>Advance journal of food science and technology >Culture Conditions Affect the Category and Production of Ubiquinones in a Recombinant Escherichia Coli with an Exogenous Decaprenyl Diphosphate Synthase
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Culture Conditions Affect the Category and Production of Ubiquinones in a Recombinant Escherichia Coli with an Exogenous Decaprenyl Diphosphate Synthase

机译：培养条件影响外源癸烯二磷酸二磷酸合酶重组大肠杆菌中泛醌的种类和产量

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摘要

Ubiquinones (UQ) are important electron transporters and play lot of important roles in most organisms. In different species, UQ was classified to be UQ-6, 7, 8, 9, 10 according to their polyprenyl side chain length. The side chain's length is determined by the enzyme named Poly-Prenyl diphosphate Synthases (PPPS). Bacteria are usually reconstructed to producing UQ-10 used in human's food additive, medicine or cosmetics, such as using decaprenyl Diphosphate Synthase (DPS) gene from R. radiobacter to substitute E. coli's octaprenyl diphosphate synthase gene, just like E. coli BL21 (ΔispB::ddsA) used in this study. It is interesting that not only in these reconstructed bacteria, but in human-being, DPS can synthesize UQ-9 besides UQ-10. The mechanism of this phenomenon is still unknown. In this study, the effects of culture conditions, including the temperature, dissolved oxygen, pH and culture medium, on the DPS characteristics in E. coli BL21 (ΔispB::ddsA) were examined. Results show that temperature greatly affects the ratio of UQ-9/UQ-10, but not the total ubiquinone's production. Increasing dissolved oxygen and protein concentration in culture medium can promote total ubiquinone's production, but not the ratio of UQ-9/UQ-10. These results may give reference for UQ-10's industrial produce and the mechanism of these conditions' effect on DPS will be discussed.
机译：泛醌（UQ）是重要的电子转运体，在大多数生物中起着许多重要的作用。在不同物种中，根据其异戊二烯基侧链长度，UQ被分类为UQ-6、7、8、9、10。侧链的长度由称为聚对苯二甲酸二磷酸合酶（PPPS）的酶确定。细菌通常会被重组以生产用于人类食品添加剂，药品或化妆品的UQ-10，例如使用放射线细菌中的癸二烯基二磷酸合酶（DPS）基因替代大肠杆菌的八烯二磷酸二磷酸合酶基因，就像大肠杆菌BL21（本研究中使用的ΔispB:: ddsA）。有趣的是，不仅在这些重组细菌中，而且在人类中，DPS除了UQ-10之外还可以合成UQ-9。这种现象的机制仍然未知。在这项研究中，研究了温度，溶解氧，pH和培养基等培养条件对大肠杆菌BL21（ΔispB:: ddsA）DPS特性的影响。结果表明，温度极大地影响了UQ-9 / UQ-10的比例，但并不影响总泛醌的产量。增加培养基中的溶解氧和蛋白质浓度可以促进总泛醌的产量，但不能提高UQ-9 / UQ-10的比例。这些结果可为UQ-10的工业生产提供参考，并讨论这些条件对DPS的作用机理。

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